High Quality Reagents Key in Therapeutic RNA Research

In a study by Pirollo et al., the potential for delivering siRNA directly to primary and metastasized tumor cells was explored. A nanosized immunoliposome-based delivery complex (scL) was developed that preferentially targets tumor cells anywhere in the body and delivers molecules useful in gene medicine, including plasmid DNA and antisense oligonucleotides. During tumor growth inhibition, animals remained healthy and continued to gain weight. The study concluded that scL may be part of the answer in therapeutic oligonucleotide development.

As the promise of oligonucleotide therapeutics ebbs and flows, one thing remains constant, studies require quality research reagents. With significant money and time invested in each experiment, you shouldn’t have to worry that reagent integrity could compromise results. TriLink offers high quality RNA synthesis with a myriad of design choices including modified RNA backbone options (2’ OMe and 2’ Fluoro), purification options (PAGE and AX-HPLC), scale options (0.2 umole up to 10 grams final yield) and over 200 modification options.

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RNA 100mers and Beyond

As research continues to demand longer and longer oligonucleotides, the TriLink Team continues to improve our chemical synthesis capabilities. Successfully preparing long oligonucleotides greatly depends on coupling efficiencies. Theoretical yield is calculated using x^y, where x is the average coupling efficiency and y is the number of couplings. Therefore a 30mer with an average coupling efficiency of 99% theoretically yields 75% product. Moving to a 100mer with the same coupling efficiency, yields 37% product. One can quickly see the need to develop improved protocols. TriLink has successfully manufactured 110mer RNA while maintaining our high quality standards and rigorous QC processes to create the products our customers need.

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Q - Do I need to have my oligonucleotide purified? If so, what method is best?

A - The need for oligonucleotide purification is dependent on a number of factors, including the type of application it will be used for and if it is modified. HPLC and PAGE are the most common purification methods. Long oligonucleotides (45mer+) do not resolve well by HPLC, therefore PAGE purification is recommended. RNA requires purification under RNase free conditions, which is most easily controlled during a PAGE purification procedure. Our sales department can assist you in determining the best purification method for your construct and requirements.

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