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“Combining CleanAmp™ dNTPs with our novel lyophilization methods creates the ultimate in high quality easy-to-use PCR assays. Inclusion of CleanAmp™ dNTPs allows us to create accurate, reproducible and simple diagnostic kits, that greatly improve the quality of clinical testing and patient care.”
Mike Bunce,
CEO and CSO
Biofortuna |
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Make Any Assay a Hot Start
CleanAmp™ dNTPs are the most versatile solution in TriLink's line of PCR enhancing products, offering a universal approach to improved Hot Start PCR. Replacement of the standard dNTPs in the PCR reaction mix with CleanAmp™ dNTPs, offers the same advantages as more costly Hot Start enzymes.
CleanAmp™ dNTPs offer precise control at the start of PCR thermal cycling by blocking nucleotide incorporation until heat activation of the dNTPs. The temperature-dependent control of DNA polymerase extension vastly reduces mis-priming, primer dimer formation and other common deleterious effects (Figure 1).
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CleanAmp™ dNTPs improve the specificity of target amplification, allowing for enhanced detection of low concentrations of template as shown in Figure 2. When standard dNTPs are employed reduced sensitivity in real-time detection is seen due to predominant primer dimer formation at lower template concentrations.
A Flexible Solution
CleanAmp™ dNTPs improve the performance of a number of thermostable DNA polymerases, allowing for greater flexibility in PCR design. The use of CleanAmp™ dNTPs in the experiment shown in Figure 3 eliminated or reduced primer dimer formation in all cases. Moreover, each DNA polymerase was able to produce the desired amplicon in higher yield, demonstrating the versatility of CleanAmp™ dNTPs.
CleanAmp™ dNTPs are available in sets as well as in a mix, providing more flexibility in your experiment design, including the ability to achieve a Hot Start reaction without the need to substitute all four dNTPs. In Figure 4, a reduction in primer dimer formation and improvement in amplicon yield is achieved by substituting only dATP and dTTP with CleanAmp™ dATP and CleanAmp™ dTTP, respectively.
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