Make Any Assay a Hot Start
CleanAmp™ dNTPs, the key component in the CleanAmp™ PCR Kit, are also
available separately. They are the most versatile solution in  TriLink's
line of PCR enhancing products, offering a universal approach to improved Hot Start PCR. Replacement of the standard dNTPs, the essential DNA polymerase substrate, with CleanAmp™ dNTPs, offers the same advantages as more costly Hot Start enzymes.
CleanAmp™ dNTPs offer precise control at the start of PCR thermal cycling by blocking nucleotide incorporation until heat activation of the dNTPs. The temperature-dependent control of DNA polymerase extension vastly reduces mis-priming, primer dimer formation and other common deleterious effects (Figure 1). CleanAmp™ dNTPs improve in the specificity of target amplification, allowing for enhanced detection of low concentrations of template as shown in Figure 2. When standard dNTPs are employed reduced sensitivity in real-time detection is seen due to predominant primer dimer formation at lower template concentrations.
A Flexible Solution
CleanAmp™ dNTPs improve the performance of a number of thermostable DNA polymerases, allowing for greater flexibility in PCR design. The use of CleanAmp™ dNTPs in the experiment shown in Figure 3 eliminated or reduced primer dimer formation in all cases. Moreover, each DNA polymerase was able to produce the desired amplicon in higher yield, demonstrating the versatility of CleanAmp™ dNTPs.
CleanAmp™ dNTPs are available in sets as well as in a mix, providing more flexibility in your experiment design, including the ability to achieve a Hot Start reaction without the need to substitute all four dNTPs. In Figure 4, a reduction in primer dimer formation and improvement in amplicon yield is achieved by substituting only dATP and dTTP with CleanAmp™ dATP and CleanAmp™ dTTP, respectively.
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