- Make Your Assay Hot Start by Simply Adding CleanAmp™ - Eliminate or Reduce Off-target Amplification
- Significantly Increase Amplicon Yield and Specificity
- Commercialize for Less with Reasonable Licensing Options
The CleanAmp™ line of products are TriLink’s innovative new approach to Hot Start PCR. We applied our expertise in modified nucleic acid chemistry to develop cost-effective solutions to common PCR problems. This advancement in Hot Start technology employs two widely ignored components of the PCR reagent mix: the primers and the dNTPs. Two distinct nucleic acid thermolabile protecting groups were identified by TriLink’s R&D team to make the CleanAmp™ technology possible.
Activation of CleanAmp™ Products occurs during the initial heat cycle of Hot Start PCR and each subsequent denaturation step, releasing just enough reagent to allow efficient amplification. By limiting the amount of active reagent during the early cycles when the target is in low concentration, CleanAmp™ significantly reduces background amplification to achieve a reduction or even an elimination of primer dimer and mis-priming. CleanAmp™ Products benefit basic applications as well as more advanced variants, such as real-time, multiplex and reverse-transcription PCR.
