- Convert Established Assays to Hot Start Assays for Pennies
- Eliminate or Reduce Off-Target Amplification
- Significantly Increase Amplicon Yield and Specificity
- Commercialize for Less with Reasonable Licensing Options
A Hot Start Assay for Less
CleanAmp™ Primers are similar to CleanAmp™ dNTPs in that they contain thermolabile chemical modifications that allow for primer-based Hot Start activation in PCR. Like CleanAmp™ dNTPs, these modifications also serve to prevent primer extension at the lower temperatures of PCR setup and manipulation. A Hot Start thermal activation step removes the modification generating the corresponding unmodified primer, which supports amplification of the desired target. CleanAmp™ Primers specifically amplify your target by eliminating formation of primer dimer (Figure 1A) and mis-priming (Figure 1B) events. Furthermore, like CleanAmp™ dNTPs, CleanAmp™ Primers eliminate the need for Hot Start DNA polymerases because they are compatible with a number of standard DNA polymerases, such as Taq.
CleanAmp™ Primers are a key choice for diagnostic kits and other established assays. In these assays CleanAmp™ Primers can be as little as $0.03 per reaction at our standard starting scale. TriLink guarantees at least 10 ODs per primer synthesis, which is enough material for approximately 6,250 reactions. TriLink offers two types of CleanAmp™ Primers that vary in the rate of thermal activation: Turbo and Precision. The different rates of release of CleanAmp™ Turbo and Precision Primer modifications can be exploited for improved results in advanced applications, such as multiplex PCR (Figure 2), reverse-transcription PCR, low copy number detection (Figure 4) and fast PCR. The performance of CleanAmp™ Primers can be applied to critical applications such as molecular diagnostics, forensics, detection of infectious agents and gene expression validation.
Maximize Your Multiplex
If you are seeking a Hot Start solution for a problematic multiplex system, CleanAmp™ Primers may be your answer. Like CleanAmp™ dNTPs, CleanAmp™ Primers will improve yield and specificity in multiplex systems. However, CleanAmp™ Primers offer higher specificity with a greater number of targets. In the systems shown in Figure 2, CleanAmp™ Turbo Primers greatly improve formation of the desired products in multiplex PCR for seven and nine targets from genomic DNA sources. The development of a robust multiplex PCR assay typically requires an iterative design process to discover primer pairs that are both specific for the targets of interest and exhibit a low level of off-target amplicon formation. With CleanAmp™ Primers minimal design optimization is required.
The reduction or elimination of nonspecific amplifications achieved with the use of CleanAmp™ Primers makes them a powerful solution for improved one-step reverse-transcription (RT) PCR performance (Figure 3). By introducing CleanAmp™ Primers only the RT Primer can elongate during the reverse transcription step of the protocol, resulting in specific amplification in a one tube, single step protocol.
Reaching New Limits of Detection
CleanAmp™ Primers also offer an advantage over CleanAmp™ dNTPs in systems hindered by low template concentrations. A 10-100 fold increase in correct amplicon formation is seen when standard primers are replaced with CleanAmp™ Precision Primers (Figure 4). CleanAmp™ Primers greatly improve these systems by reducing or eliminating the off-target amplifications that compete with the desired amplicon. CleanAmp™ Primers have utility in many high-sensitivity downstream applications, such as single molecule detection.
Product Details
CleanAmp™ Primers are available in two forms that differ in the rate of thermal activation. See table below for application based benefits. CleanAmp™ Primers are supplied cartridge purified. However, for those applications where PCR performance is of the utmost importance, we can RP-HPLC purify your CleanAmp™ Primers on a custom quote basis. Each primer undergoes quality control analysis by PAGE, MS and RP-HPLC to ensure high quality. CleanAmp™ Primers 15-40 bases in length are $250/pair. We guarantee at least a 10 OD final yield from every synthesis.
CleanAmp™ Amidites
If you have oligonucleotide synthesis capabilities in-house, you may prefer making your own CleanAmp™ Primers. CleanAmp™ Amidites can be ordered online, by phone, as well as through Glen Research.
