TriLink RNA transcript service advantages:
Custom tailored service to meet specific application or program needs from template amplification to a wide variety of modifications, treatments and purification options.
Modified NTP manufacturing and technical expertise mean high quality, unique products.
Wide range of synthesis scales yielding µgrams to hundreds of milligrams.
Fully traceable documentation system.
Areas of research supported:
Stem Cell Reprogramming
Custom qPCR Standards
Transient Zinc Finger Nuclease Expression
Gene Therapy Research
RNA Structural Studies and RNase Protection Assays
Contact us today to discuss your project. |
|
 TriLink mRNA Transfection of HEK-293 Cells Leads to Robust Enhanced Green Fluorescent Protein Expression (eGFP): Capped and polyadenylated mRNAs that were fully substituted with 5-methylcytidine and pseudouridine were synthesized by T7 RNA polymerase transcription in the presence of anti-reverse cap analog (ARCA). Residual triphosphate was removed by phosphatase treatment. HEK-293 cells were transfected with 800 ng of eGFP using Lipofectamine 2000 (Invitrogen). Cells were imaged by brightfield (left image) and fluorescence microscopy (right image) at 48 hours. Fluorescence activated cell sorting indicated >95% eGFP positive cells (data not shown). Image courtesy of Jiehua Zhou, John Rossi laboratory, City of Hope. |
