| Standard Oligonucleotide Synthesis |
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| DNA OLIGOS | RNA OLIGOS | OTHER BACKBONES |
| DNA (2' Deoxy) Oligonucleotides[PER BASE PRICING] ^top |
| Our standard DNA synthesis ranges from 0.2 umol to 15 umol, in both phosphodiester and phosphorothioate backbones. We offer an extensive selection of oligo modifications, such as linkers, spacers, dyes and modified bases. For larger synthesis scales (yields of 50 mg to 10 g) please refer to our mid-scale services. |
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| Phosphodiester |
| 0.2 umole scale |
$2.00 |
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| 1.0 umole scale |
$3.00 |
| 15 umole scale |
$15.00 |
| $40 minimum |
| Phosphorothioate |
| 0.2 umole scale |
$4.50 |
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| 1.0 umole scale |
$6.00 |
| 15 umole scale |
$20.00 |
| $50 minimum |
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| 2' OH RNA and 2' O-Methyl RNA Oligonucleotides[PER BASE PRICING] ^top |
Both 2'OH RNA and 2'O-Methyl RNA are synthesized at TriLink. Since the chemistries are different, different pricing and purifications apply to each. Both 2'OH and 2'O-Methyl RNA oligonucleotides can be made with phosphorothioate linkages at no extra charge.
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| 2' OH RNA |
| 0.2 umole scale |
$12.50 |
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| 1.0 umole scale |
$17.50 |
| 15 umole scale |
$75.00 |
| $100 minimum |
| 2' O-Methyl RNA |
| 0.2 umole scale |
$8.00 |
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| 1.0 umole scale |
$12.00 |
| 15 umole scale |
$50.00 |
| $50 minimum |
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| Other Backbone and 2' Ribose Modifications[PER BASE PRICING] ^top |
Methylphosphonate Oligonucleotides
Methylphosphonate is one of the few neutrally charged backbones. This modification is most commonly used to protect the termini of oligonucleotides from enzymatic degradation.
*Minimum refers only to the portion of the oligonucleotide with the methylphosphonate modification.
2' Fluoro Oligonucleotides
The main applications of this modification are ribozyme development (1,3), to increase Tm (2,6,7), aptamer selection (4,5), and nuclease resistance (7). Oligonucleotides containing 2’ fluoros have been reported to increase DNA-DNA Tm by 1.3°C per insertion (6).
- Fell, et al. (1997) Antisense and NAD 7, 319-326.
- Pagratis, et al. (1997) Nature Biotechnology 15, 68-73.
- Ito, et al (1999) Nucl. Acids Symp. Ser 42, 277-278.
- Pat. No. 5,475,096, Nexstar.
- Kubik, et al (1997) J. Immunol. 159, 259-267.
- Sabahi, et al (2001) Nucleic Acids Rsrch 29, 2163-2170.
- Kawasaki, et al (1993) J. Medicinal Chem 36, 831-841.
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| Methylphosphonate |
| 0.2 umole scale |
N/A |
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| 1.0 umole scale |
$35.00 |
| 15 umole scale |
$125.00 |
| $150 minimum* |
| 2' Fluoro RNA (C
and U) |
| 0.2 umole scale |
N/A |
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| 1.0 umole scale |
$50.00 |
| 15 umole scale |
$250.00 |
| $100 minimum |
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