Our standard DNA synthesis ranges from 0.2 µmole to 15 µmole, in both phosphodiester and phosphorothioate backbones. We offer an extensive selection of oligo modifications, such as linkers, spacers, dyes and modified bases.

For larger synthesis scales please refer to our mid-scale services or request a custom quotation.

Our standard RNA synthesis services range from 0.2 µmole to 15 µmole scale, in both 2' OH RNA and 2' O-Methyl RNA backbones. Both 2' OH and 2' O-Methyl RNA oligonucleotides can be made with phosphorothioate linkages at no extra charge. We offer an extensive selection of oligo modifications, such as linkers, spacers, dyes and modified bases.

For larger synthesis scales please request a custom quotation. Set pricing for mid-scale RNA oligonucleotide synthesis is not available, because the yield and quality of these syntheses is highly dependent on sequence.

Methylphosphonate Oligonucleotides
This molecule has a number of unusual properties. It is one of the few neutrally charged backbones that is readily available. This modification is most commonly used to protect the termini of oligonucleotides from enzymatic degradation. One disadvantage is that the racemic form of this modification reduces the ability of the oligonucleotide to hybridize to its target.
*This minimum refers only to the portion of the oligonucleotide with the methylphosphonate modification. This is to cover the additional deprotection processing required for this modification.

2' Fluoro RNA Oligonucleotides
The main applications of this modification are ribozyme development (1,3), to increase Tm (2,6,7), aptamer selection (4,5), and nuclease resistance (7). Oligonucleotides containing 2’ fluoros have been reported to increase DNA-DNA Tm by 1.3°C per insertion (6).

1. Fell, et al. (1997) Antisense and NAD 7, 319-326.
2. Pagratis, et al. (1997) Nature Biotechnology 15, 68-73.
3. Ito, et al (1999) Nucl. Acids Symp. Ser 42, 277-278.
4. Pat. No. 5,475,096, Nexstar.
5. Kubik, et al (1997) J. Immunol. 159, 259-267.
6. Sabahi, et al (2001) Nucleic Acids Rsrch 29, 2163-2170.
7. Kawasaki, et al (1993) J. Medicinal Chem 36, 831-841.