Molecular Beacons A novel diagnostic assay utilizing oligonucleotides with a fluorescent dye at one terminus and a quenching dye at the other. Molecular beacons form a hairpin when not hybridized to a target, thus placing the 5' and 3' termini, and their attached dyes, in close proximity. The fluorescent dye is quenched, yielding no signal. However, upon hybridization of the probe to the target, the dyes are separated and fluorescence is detected.
5' Fluorophore
0.2 µmole Scale Synthesis
1.0 µmole Scale Synthesis
without a license*
with a license*
expected yield
without a license*
with a license*
expected yield
Carboxy-X-Rhodamine™
$600
$490
1-3 ODs
$700
$575
3-15 ODs
Coumarin™
$600
$490
1-3 ODs
$700
$575
3-15 ODs
Cy3™ (SE)
$600
$490
1-3 ODs
$700
$575
3-15 ODs
Cy3.5™ (SE)
$750
$615
1-3 ODs
$850
$700
3-15 ODs
Cy5™ (SE)
$600
$490
1-3 ODs
$700
$575
3-15 ODs
Cy5.5™ (SE)
$750
$615
1-3 ODs
$850
$700
3-15 ODs
6-FAM
$450
$370
3-5 ODs
$550
$450
10-20 ODs
HEX (SE)
$450
$370
3-5 ODs
$550
$450
10-20 ODs
Rhodamine Green™
$600
$490
1-3 ODs
$700
$575
3-15 ODs
Rhodamine Red™
$600
$490
1-3 ODs
$700
$575
3-15 ODs
TAMRA
$600
$490
1-3 ODs
$700
$575
3-15 ODs
TET
$450
$370
3-5 ODs
$550
$450
10-15 ODs
Texas Red®
$600
$490
1-3 ODs
$700
$575
3-15 ODs
NOTES: Pricing is for DABCYL, BHQ-1 or BHQ-2 3' quenchers.
*Molecular Beacons are sold under license from thr Public Health Research, Inst. of New York and may only be used for research purposes. No other license is granted or implied with the purchase of this product. Our standard pricing includes an 18% surcharge to cover royalty fees required by the licensor. If you have a license for the use of molecular beacons, please let us know when you place an order and you will receive an automatic 18% discount. Neither TriLink nor PHRI warrants this product beyond replacement cost for any reason.
Disclaimer of License Statement for Molecular Beacons Products: The 5' Nuclease detection assay and other homogeneous amplification methods used in connection with Polymerase Chain Reaction ("PCR") Process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd ("Roche"). Use of these methods requires a license. No license under these patents, which include but are not limited to United States Patent Nos. 5,210,015, 5,487,972, 5,804,375, and 5,994,056 to use the 5' Nuclease Assay or any Roche patented homogeneous amplification process is conveyed expressly or by implication to the purchaser by the purchase of any TriLink BioTechnologies, Inc. PCR-related product. Purchasers of these products must obtain a license to use the 5' Nuclease or Homogeneous PCR process before performing PCR. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Licensing Specialist at (510) 814-2984, Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California, 94501.
