Light-Activated OligonucleotidesFor Gene Regulation, PCR, Mutagenesis & More! Coming Soon! Please inquire for a quotation and availability of early access material.
These uniquely modified oligonucleotides are useful in a variety of applications, including:
Introduce new mutations into plasmid DNA: single base pair mutation, stop codon or deletion of entire sequence, etc.
Light-activated oligonucleotides contain photolabile nucleobase modifications, nitropiperonyloxymethyl (NPOM), to one or more of
the thymidines. The presence of the NPOM-caging group interrupts hybridization by sterically blocking Watson-Crick base pairing.
Light activation by UV irradiation specifically removes the NPOM-caging group without damaging the oligonucleotide.
The ability to regulate DNA:DNA or DNA:RNA hybridization with light provides a powerful alternative for assay activation.
Figure adapted from reference 3.
The NPOM-caging group technology was invented in the Deiters Lab at North Carolina State University.
