2',3'-Dideoxycytidine-5'-Triphosphate

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N-4005
ddCTP, Zalcitabine triphosphate
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SKU Unit Size Price Qty
N-4005-1 1 µmole
$32.00
N-4005-5 5 µmole
$134.00
N-4005-10 10 µmole
$243.00
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Description

2',3'-Dideoxycytidine-5'-Triphosphate (ddCTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddCTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. "I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results." Michal Legiewicz Research Scientist Yale University

Product Details

Catalog No N-4005
Purity ≥95% by AX-HPLC
Extinction Coefficient 9,100 Lmol-1cm-1 at 271 nm
Molecular Formula C9H16N3O12P3 (free acid)
Molecular Weight 451.10 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) ddCTP, Zalcitabine triphosphate
Application Aptamers
Backbone 5'-Triphosphate
Base Analog(s) Cytidine
Nucleotide Category Sugar Modified

Technical Documents




Intellectual Property

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References

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Vooradi, Venkataramana; Romano, Louis J. . Effect ofN-2-Acetylaminofluorene and 2-Aminofluorene Adducts on DNA Binding and Synthesis by Yeast DNA Polymerase

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