2',3'-Dideoxynucleotide Set

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K-1004
ddNTP Set, DNA Chain Terminator Set
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Catalog # Unit Size Price Qty
K-1004 1 µmole each
$116.00
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Description

Contains 1.0 µmole each of 4 nucleotides:

Chain terminators are sugar modified nucleoside triphosphates, where the 2' and 3' hydroxyl groups are absent. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddNTPs are used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.

Product Details

Catalog No K-1004
Components 2',3'-Dideoxyadenosine-5'-Triphosphate, 2',3'-Dideoxyguanosine-5'-Triphosphate, 2',3'-Dideoxythymidine-5'-Triphosphate, 2',3'-Dideoxycytidine-5'-Triphosphate
Recommended Storage -20°C or below
Application Aptamers, PCR & RT-PCR
Backbone Phosphodiester
Base Analog(s) Adenosine, Cytidine, Guanosine, Thymidine
Sugar Type(s) 2'-3'-Dideoxy
Other Name(s) ddNTP Set, DNA Chain Terminator Set


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