4-Thiouridine is useful for UV photocrosslinking studies to identify sites of ribonucleoprotein-RNA interactions and to crosslink sites containing RNA secondary and tertiary structure. Incorporation of 4-Thiouridine allows for specific crosslinking at longer wavelengths, typically between 320 and 360 nm, which results in minimal RNA damage compared to standard shortwave UV light. 4-Thiouridine-5'-Triphosphate can also be incorporated into RNA to allow specific and efficient tagging of the modified base with sulfur-reactive methylthiosulfonate-activated labels.
"The 4-Thio-UTP I had ordered from TriLink worked like a charm."
Vaishnavi Rajagopal Post-doctoral Fellow
Johns Hopkins University School of Medicine
blank "We were impressed with the timing and the quality of the 4-Thio-UTP that we ordered. Obviously it is of utmost importance to our research that we are able to rely on companies such as TriLink to provide quality products when we require them."
Christine Woltjen Research Associate
Ohio State University
Product Details
Catalog No
N-1025
Purity
≥90% by AX-HPLC
Extinction Coefficient
16,526 Lmol-1cm-1 at 331 nm
Molecular Formula
C9H15N2O14P3S (free acid)
Molecular Weight
500.20 g/mole (free acid)
Salt Form
Li+
Concentration
100 mM
Buffer
H2O
Recommended Storage
-20°C or below
Other Name(s)
4-Thio-UTP, Thio UTP
Application
Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
