4-Thiouridine-5'-Triphosphate - (N-1025)
4-Thio-UTP, Thio UTP
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N-1025
Description
4-Thiouridine is useful for UV photocrosslinking studies to identify sites of ribonucleoprotein-RNA interactions and to crosslink sites containing RNA secondary and tertiary structure. Incorporation of 4-Thiouridine allows for specific crosslinking at longer wavelengths, typically between 320 and 360 nm, which results in minimal RNA damage compared to standard shortwave UV light. 4-Thiouridine-5'-Triphosphate can also be incorporated into RNA to allow specific and efficient tagging of the modified base with sulfur-reactive methylthiosulfonate-activated labels.
"The 4-Thio-UTP I had ordered from TriLink worked like a charm."
Vaishnavi Rajagopal Post-doctoral Fellow
Johns Hopkins University School of Medicine
blank "We were impressed with the timing and the quality of the 4-Thio-UTP that we ordered. Obviously it is of utmost importance to our research that we are able to rely on companies such as TriLink to provide quality products when we require them."
Christine Woltjen Research Associate
Ohio State University
Product details
| Catalog No | N-1025 |
|---|---|
| Purity | ≥90% by AX-HPLC |
| Extinction Coefficient | 16,526 Lmol-1cm-1 at 331 nm |
| Molecular Formula | C9H15N2O14P3S (free acid) |
| Molecular Weight | 500.20 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | 4-Thio-UTP, Thio UTP |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Uridine |
| Sugar Type(s) | RNA |
| Nucleotide Category | Base Modified RNA |
Technical documents
N-1025 Safety Data Sheet
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Certificate of analysis
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Intellectual property
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
References
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- Park, Euisun; Baron, Rose; Landgraf, Ralf . Higher-Order Association States of Cellular ERBB3 Probed with Photo-Cross-Linkable Aptamersâ€ÂÂ
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- Wang, Xueyin; Goodrich, Karen J.; Gooding, Anne R.; Naeem, Haroon; Archer, Stuart; Paucek, Richard D.; Youmans, Daniel T.; Cech, Thomas R.; Davidovich, Chen . Targeting of Polycomb Repressive Complex 2 to RNA by Short Repeats of Consecutive Guanines.
- Schofield, Jeremy A.; Duffy, Erin E.; Kiefer, Lea; Sullivan, Meaghan C.; Simon, Matthew D. . TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding.
- Hallé, François; Fin, Andrea; Rovira, Alexander R.; Tor, Yitzhak . Emissive Synthetic Cofactors: Enzymatic Interconversions of (tz) A Analogues of ATP, NAD(+) , NADH, NADP(+) , and NADPH.
- Shubham, Shambhavi; Hoinka, Jan; Banerjee, Soma; Swanson, Emma; Dillard, Jacob A.; Lennemann, Nicholas J.; Przytycka, Teresa M.; Maury, Wendy; Nilsen-Hamilton, Marit . A 2'FY-RNA Motif Defines an Aptamer for Ebolavirus Secreted Protein.
- Barbieri, E;Hill, C;Quesnel-Valli . Rapid and Scalable Profiling of Nascent RNA with fastGRO
- Fujiwara, R; . Structral and Biochemical Insights Into the Transition from Transcription Initiation to Elongation
