7-Deaza-2'-deoxyguanosine-5'-Triphosphate - (N-2011)
7-Deaza-dGTP
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N-2011
Description
This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.
Product details
| Catalog No | N-2011 |
|---|---|
| Purity | ≥95% by AX-HPLC |
| Extinction Coefficient | 10,069 Lmol-1cm-1 at 259 nm |
| Molecular Formula | C11H17 N4O13P3 (free acid) |
| Molecular Weight | 506.00 g/mole (free acid) |
| Salt Form | Li+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | 7-Deaza-dGTP |
| Application | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies |
| Backbone | 5'-Triphosphate |
| Base Analog(s) | Guanosine |
| Sugar Type(s) | DNA |
| Nucleotide Category | Base Modified DNA |
Technical documents
N-2011 Safety Data Sheet
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Intellectual property
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References
- Mizusawa, S.; Nishimura, S.; Seela, F. . Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.
- Maloney, Bryan; Ray, Balmiki; Hayden, Elizabeth P.; Nurnberger, John I. Jr; Lahiri, Debomoy K. . Development and validation of the high-quality 'rapid method for swab' to genotype the HTTLPR serotonin transporter (SLC6A4) promoter polymorphism.
- Musso, Marco; Bocciardi, Renata; Parodi, Sara; Ravazzolo, Roberto; Ceccherini, Isabella . Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences.
- Pivoňková, Hana; Horáková, Petra; Fojtová, Miloslava; Fojta, Miroslav . Direct Voltammetric Analysis of DNA Modified with Enzymatically Incorporated 7-Deazapurines
- Wei, Maochen; Deng, Jing; Feng, Kun; Yu, Boyang; Chen, Yijun . Universal Method Facilitating the Amplification of Extremely GC-Rich DNA Fragments from Genomic DNA
- Deféver, Thibaut; Druet, Michel; Rochelet-Dequaire, Murielle; Joannes, Martine; Grossiord, Céline; Limoges, Benoit; Marchal, Damien . Real-Time Electrochemical Monitoring of the Polymerase Chain Reaction by Mediated Redox Catalysis
- DeCarlo, Lindsey; Gowda, A. S. Prakasha; Suo, Zucai; Spratt, Thomas E. . Formation of purine-purine mispairs by Sulfolobus solfataricus DNA polymerase IV.
- Zhang, Xuemei; Motea, Edward; Lee, Irene; Berdis, Anthony J. . Replication of a Universal Nucleobase Provides Unique Insight into the Role of Entropy during DNA Polymerization and Pyrophosphorolysis
- Shore, Sabrina; Paul, Natasha . Robust PCR amplification of GC-rich targets with Hot Start 7-deaza-dGTP
- Patro, Jennifer N.; Urban, Milan; Kuchta, Robert D. . Interaction of Human DNA Polymerase and DNA Polymerase I fromBacillus stearothermophiluswith Hypoxanthine and 8-Oxoguanine Nucleotides
- Wanrooij, Paulina H.; Uhler, Jay P.; Shi, Yonghong; Westerlund, Fredrik; Falkenberg, Maria; Gustafsson, Claes M. . A hybrid G-quadruplex structure formed between RNA and DNA explains the extraordinary stability of the mitochondrial R-loop.
- Stevens, Aaron J.; Kennedy, Hannah L.; Kennedy, Martin A. . Fluorescence Methods for Probing G-Quadruplex Structure in Single- and Double-Stranded DNA.
- Hollenstein, Marcel; Damha, Masad J. . Rolling Circle Amplification with Chemically Modified Nucleoside Triphosphates.
- Gowda, A. S. Prakasha; Krzeminski, Jacek; Amin, Shantu; Suo, Zucai; Spratt, Thomas E. . Mutagenic Replication of N(2)-Deoxyguanosine Benzo[a]pyrene Adducts by Escherichia coli DNA Polymerase I and Sulfolobus solfataricus DNA Polymerase IV.
- Hollenstein, M;Damha, MJ; . Rolling Circle Amplification with Chemically Modified Nucleoside Triphosphates
