7-Deaza-2'-deoxyguanosine-5'-Triphosphate

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N-2011
7-Deaza-dGTP
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SKU Unit Size Price Qty
N-2011-1 1 µmole
$70.00
N-2011-5 5 µmole
$331.00
N-2011-10 10 µmole
$552.00
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Description

This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.

Product Details

Catalog No N-2011
Purity ≥95% by AX-HPLC
Extinction Coefficient 10,069 Lmol-1cm-1 at 259 nm
Molecular Formula C11H17 N4O13P3 (free acid)
Molecular Weight 506.00 g/mole (free acid)
Salt Form Li+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) 7-Deaza-dGTP
Application Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies
Backbone 5'-Triphosphate
Base Analog(s) Guanosine
Sugar Type(s) DNA
Nucleotide Category Base Modified DNA

Technical Documents




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References

Mizusawa, S.; Nishimura, S.; Seela, F. . Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.

Maloney, Bryan; Ray, Balmiki; Hayden, Elizabeth P.; Nurnberger, John I. Jr; Lahiri, Debomoy K. . Development and validation of the high-quality 'rapid method for swab' to genotype the HTTLPR serotonin transporter (SLC6A4) promoter polymorphism.

Musso, Marco; Bocciardi, Renata; Parodi, Sara; Ravazzolo, Roberto; Ceccherini, Isabella . Betaine, dimethyl sulfoxide, and 7-deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences.

Pivoňková, Hana; Horáková, Petra; Fojtová, Miloslava; Fojta, Miroslav . Direct Voltammetric Analysis of DNA Modified with Enzymatically Incorporated 7-Deazapurines

Wei, Maochen; Deng, Jing; Feng, Kun; Yu, Boyang; Chen, Yijun . Universal Method Facilitating the Amplification of Extremely GC-Rich DNA Fragments from Genomic DNA

Deféver, Thibaut; Druet, Michel; Rochelet-Dequaire, Murielle; Joannes, Martine; Grossiord, Céline; Limoges, Benoit; Marchal, Damien . Real-Time Electrochemical Monitoring of the Polymerase Chain Reaction by Mediated Redox Catalysis

DeCarlo, Lindsey; Gowda, A. S. Prakasha; Suo, Zucai; Spratt, Thomas E. . Formation of purine-purine mispairs by Sulfolobus solfataricus DNA polymerase IV.

Zhang, Xuemei; Motea, Edward; Lee, Irene; Berdis, Anthony J. . Replication of a Universal Nucleobase Provides Unique Insight into the Role of Entropy during DNA Polymerization and Pyrophosphorolysis

Shore, Sabrina; Paul, Natasha . Robust PCR amplification of GC-rich targets with Hot Start 7-deaza-dGTP

Patro, Jennifer N.; Urban, Milan; Kuchta, Robert D. . Interaction of Human DNA Polymerase and DNA Polymerase I fromBacillus stearothermophiluswith Hypoxanthine and 8-Oxoguanine Nucleotides

Wanrooij, Paulina H.; Uhler, Jay P.; Shi, Yonghong; Westerlund, Fredrik; Falkenberg, Maria; Gustafsson, Claes M. . A hybrid G-quadruplex structure formed between RNA and DNA explains the extraordinary stability of the mitochondrial R-loop.

Stevens, Aaron J.; Kennedy, Hannah L.; Kennedy, Martin A. . Fluorescence Methods for Probing G-Quadruplex Structure in Single- and Double-Stranded DNA.

Hollenstein, Marcel; Damha, Masad J. . Rolling Circle Amplification with Chemically Modified Nucleoside Triphosphates.

Gowda, A. S. Prakasha; Krzeminski, Jacek; Amin, Shantu; Suo, Zucai; Spratt, Thomas E. . Mutagenic Replication of N(2)-Deoxyguanosine Benzo[a]pyrene Adducts by Escherichia coli DNA Polymerase I and Sulfolobus solfataricus DNA Polymerase IV.