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ARCA - (N-7003)

Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G
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N-7003
Product SKU Unit Size Price Qty
N-7003
N-7003-1 1 µmole
$150.00
N-7003-5 5 µmole
$650.00
N-7003-10 10 µmole
$1,278.00
Description
Trusted TriLink quality, exceptional price. A key step in cellular mRNA processing is the addition of a 5’ cap structure, which is a 5'-5' triphosphate linkage between the 5' end of the RNA and a guanosine nucleotide. The cap is methylated enzymatically at the N-7 position of the guanosine to form mature mCAP. When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs. If mCAP (pre-methylated CAP) is used, only half of the asymmetrical mCAP will insert in the functional orientation. ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently. TriLink is the least expensive source of high quality ARCA. We also offer CAP and mCAP for more traditional capping reactions.
Product details
Catalog No N-7003
Purity ≥95% by AX-HPLC
Extinction Coefficient 21,000 Lmol-1cm-1 at 254 nm
Molecular Formula C22H32N10O18P3 (free acid)
Molecular Weight 817.40 g/mole (free acid)
Salt Form NH4+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G
Application CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases
Backbone 5'-5'-Triphosphate
Base Analog(s) Guanosine
Nucleotide Category Cap analogs
Cap Analogs Other
Technical documents

N-7003 Safety Data Sheet

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Intellectual property

ARCA (Anti-Reverse Cap Analog) This material and products incorporating it are sold under license from the Board of Supervisors of Louisiana State University and Agricultural and Mechanical College and Dr. Edward Darzynkiewicz and may be used for research use only, excluding any use in humans or for any diagnostic or therapeutic purpose. U.S. Patent 7,074,596.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission from TriLink No license under any patent or other intellectual property right of TriLink or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.

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