ARCA

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N-7003
Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G
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SKU Unit Size Price Qty
N-7003-1 1 µmole
$134.00
N-7003-5 5 µmole
$618.00
N-7003-10 10 µmole
$1,137.00
Request Bulk Pricing

Description

Trusted TriLink quality, exceptional price. A key step in cellular mRNA processing is the addition of a 5’ cap structure, which is a 5'-5' triphosphate linkage between the 5' end of the RNA and a guanosine nucleotide. The cap is methylated enzymatically at the N-7 position of the guanosine to form mature mCAP. When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs. If mCAP (pre-methylated CAP) is used, only half of the asymmetrical mCAP will insert in the functional orientation. ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently. TriLink is the least expensive source of high quality ARCA. We also offer CAP and mCAP for more traditional capping reactions.

Product Details

Catalog No N-7003
Purity ≥95% by AX-HPLC
Extinction Coefficient 21,000 Lmol-1cm-1 at 254 nm
Molecular Formula C22H32N10O18P3 (free acid)
Molecular Weight 817.40 g/mole (free acid)
Salt Form NH4+
Concentration 100 mM
Buffer H2O
Recommended Storage -20°C or below
Other Name(s) Anti Reverse Cap Analog, 3´-O-Me-m7G(5')ppp(5')G
Application CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases
Backbone 5'-5'-Triphosphate
Base Analog(s) Guanosine
Nucleotide Category Cap analogs

Technical Documents




Intellectual Property

ARCA (Anti-Reverse Cap Analog) This material and products incorporating it are sold under license from the Board of Supervisors of Louisiana State University and Agricultural and Mechanical College and Dr. Edward Darzynkiewicz and may be used for research use only, excluding any use in humans or for any diagnostic or therapeutic purpose. U.S. Patent 7,074,596.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.



References

Karikó, Katalin; Buckstein, Michael; Ni, Houping; Weissman, Drew . Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA.

Anderson, Bart R.; Muramatsu, Hiromi; Nallagatla, Subba R.; Bevilacqua, Philip C.; Sansing, Lauren H.; Weissman, Drew; Karikó, Katalin . Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation.

Anderson, Bart R.; Muramatsu, Hiromi; Jha, Babal K.; Silverman, Robert H.; Weissman, Drew; Karikó, Katalin . Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L.

Peng, Zheng-Hong; Sharma, Vivek; Singleton, Scott F.; Gershon, Paul D. . Synthesis and application of a chain-terminating dinucleotide mRNA cap analog.

Filipowicz, W. . Functions of the 5,-terminal m7G cap in eukaryotic mRNA.

Banerjee, A. K. . 5'-terminal cap structure in eucaryotic messenger ribonucleic acids.

Miura, K. . The cap structure in eukaryotic messenger RNA as a mark of a strand carrying protein information.

Zan-Kowalczewska, M.; Bretner, M.; Sierakowska, H.; Szczesna, E.; Filipowicz, W.; Shatkin, A. J. . Removal of 5'-terminal m7G from eukaryotic mRNAs by potato nucleotide pyrophosphatase and its effect on translation.

Konarska, M. M.; Padgett, R. A.; Sharp, P. A. . Recognition of cap structure in splicing in vitro of mRNA precursors.

Rosenberg, M.; Paterson, B. M. . Efficient cap-dependent translation of polycistronic prokaryotic mRNAs is restricted to the first gene in the operon.

Warren, Luigi; Ni, Yuhui; Wang, Jiwu; Guo, Xirong . Feeder-free derivation of human induced pluripotent stem cells with messenger RNA.

Uchida, Satoshi; Itaka, Keiji; Uchida, Hirokuni; Hayakawa, Kentaro; Ogata, Toru; Ishii, Takehiko; Fukushima, Shigeto; Osada, Kensuke; Kataoka, Kazunori . In vivo messenger RNA introduction into the central nervous system using polyplex nanomicelle.

Guo, Xing Rong; Wang, Xiao Li; Li, Man Chol; Yuan, Ya Hong; Chen, Yun; Zou, Dan Dan; Bian, Liu Jiao; Li, Dong Sheng . PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro.

Wang, Xiao Li; Hu, Pei; Guo, Xing Rong; Yan, Ding; Yuan, Yahong; Yan, Shi Rong; Li, Dong Sheng . Reprogramming human umbilical cord mesenchymal stromal cells to islet-like cells with the use of in vitro–synthesized pancreatic-duodenal homebox 1 messenger RNA

Kenney, Scott P.; Meng, Xiang-Jin . The lysine residues within the human ribosomal protein S17 sequence naturally inserted into the viral nonstructural protein of a unique strain of hepatitis E virus are important for enhanced virus replication.

Almåsbak, H.; Walseng, E.; Kristian, A.; Myhre, M. R.; Suso, E. M.; Munthe, L. A.; Andersen, J. T.; Wang, M. Y.; Kvalheim, G.; Gaudernack, G.; Kyte, J. A. . Inclusion of an IgG1-Fc spacer abrogates efficacy of CD19 CAR T cells in a xenograft mouse model.

Lee, Kunwoo; Yu, Pengzhi; Lingampalli, Nithya; Kim, Hyun Jin; Tang, Richard; Murthy, Niren . Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells.

Koblas, Tomas; Leontovyc, Ivan; Loukotova, Sarka; Kosinova, Lucie; Saudek, Frantisek . Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors.

Kondrat, Jason; Sultana, Nishat; Zangi, Lior . Synthesis of Modified mRNA for Myocardial Delivery.

Rauch, Susanne; Lutz, Johannes; Kowalczyk, Aleksandra; Schlake, Thomas; Heidenreich, Regina . RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines.

Sultana, Nishat; Magadum, Ajit; Hadas, Yoav; Kondrat, Jason; Singh, Neha; Youssef, Elias; Calderon, Damelys; Chepurko, Elena; Dubois, Nicole; Hajjar, Roger J.; Zangi, Lior . Optimizing Cardiac Delivery of Modified mRNA.

Inderberg, Else M.; Wälchli, Sébastien; Myhre, Marit R.; Trachsel, Sissel; Almåsbak, Hilde; Kvalheim, Gunnar; Gaudernack, Gustav . T cell therapy targeting a public neoantigen in microsatellite instable colon cancer reduces in vivo tumor growth.

Dastagir, Shamael R.; Postigo-Fernandez, Jorge; Xu, Chunliang; Stoeckle, James H.; Firdessa-Fite, Rebuma; Creusot, Rémi J. . Efficient Presentation of Multiple Endogenous Epitopes to Both CD4(+) and CD8(+) Diabetogenic T Cells for Tolerance.

Ehret, Fabian; Zhou, Cun Yu; Alexander, Seth C.; Zhang, Dongyang; Devaraj, Neal K. . Site-Specific Covalent Conjugation of Modified mRNA by tRNA Guanine Transglycosylase.

Leontovyc, Ivan; Habart, David; Loukotova, Sarka; Kosinova, Lucie; Kriz, Jan; Saudek, Frantisek; Koblas, Tomas . Synthetic mRNA is a more reliable tool for the delivery of DNA-targeting proteins into the cell nucleus than fusion with a protein transduction domain.

Moffett, H. F.; Coon, M. E.; Radtke, S.; Stephan, S. B.; McKnight, L.; Lambert, A.; Stoddard, B. L.; Kiem, H. P.; Stephan, M. T. . Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers.

Walseng, Even; Köksal, Hakan; Sektioglu, Ibrahim M.; Fåne, Anne; Skorstad, Gjertrud; Kvalheim, Gunnar; Gaudernack, Gustav; Inderberg, Else Marit; Wälchli, Sébastien . A TCR-based Chimeric Antigen Receptor.

Li, Bin; Zhao, Weiyu; Luo, Xiao; Zhang, Xinfu; Li, Chenglong; Zeng, Chunxi; Dong, Yizhou . Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency.

Yanez Arteta, Marianna; Kjellman, Tomas; Bartesaghi, Stefano; Wallin, Simonetta; Wu, Xiaoqiu; Kvist, Alexander J.; Dabkowska, Aleksandra; Székely, Noémi; Radulescu, Aurel; Bergenholtz, Johan; Lindfors, Lennart . Successful reprogramming of cellular protein production through mRNA delivered by functionalized lipid nanoparticles.

Suknuntha, Kran; Tao, Lihong; Brok-Volchanskaya, Vera; D'Souza, Saritha S.; Kumar, Akhilesh; Slukvin, Igor . Optimization of Synthetic mRNA for Highly Efficient Translation and its Application in the Generation of Endothelial and Hematopoietic Cells from Human and Primate Pluripotent Stem Cells.

De Scheerder, Louis; Sparén, Anders; Nilsson, Gunilla A.; Norrby, Per-Ola; Örnskov, Eivor . Designing flexible low-viscous sieving media for capillary electrophoresis analysis of ribonucleic acids.

Singh, Raman Deep; Hillestad, Matthew L.; Livia, Christopher; Li, Mark; Alekseev, Alexey E.; Witt, Tyra A.; Stalboerger, Paul G.; Yamada, Satsuki; Terzic, Andre; Behfar, Atta . M(3)RNA Drives Targeted Gene Delivery in Acute Myocardial Infarction.

Magadum, Ajit; Singh, Neha; Kurian, Ann Anu; Sharkar, Mohammad Tofael Kabir; Chepurko, Elena; Zangi, Lior . Ablation of a Single N-Glycosylation Site in Human FSTL 1 Induces Cardiomyocyte Proliferation and Cardiac Regeneration.