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Antibody-Oligonucleotide All-in-One Conjugation Kit

Proven kits for ImmunoPCR, PLA, ECPA, and more.

No chromatography required!

The first referenced, easy-to-use kit to conjugate antibodies with oligos

The Antibody-Oligonucleotide All-in-One Conjugation Kit offers an innovative TurboLink catalyzed conjugation based on Chromalink™ technology to prepare antibody-oligonucleotide conjugates without chromatography. This novel kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation Kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

Download White Paper: Antibody-Oligonucleotide Conjugate Preparation

How it works
Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:

  1. Amine-modified, 20 to 60-mer oligonucleotide is modified using an excess of the Sulfo-S-4FB linker. This reactive NHS-ester incorporates a 4FB (aromatic aldehyde functional group, formylbenzamide) at the desired terminus of the oligonucleotide.
  2. Polyclonal or monoclonal antibody (100 g) is modified using the S-HyNic linker. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazino-nicotinamide) onto the antibody.
  3. The two modified biomolecules are mixed together in the presence of the TurboLink catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds, followed by magnetic-affinity, solid phase purification.
  4. The antibody-oligonucleotide conjugate is ready for use in the most demanding and sensitive applications.

Advantages

  • Simple and Easy to Use Requires only micropipette, microcentrifuge, and UV spectrophotometer
  • Automated Calculations Calculator with fully integrated input/output provided
  • High Yield 3050% yield based on starting antibody
  • High Purity >95% purity without chromatographic purification
  • High Stability Conjugates are stable for >1 year
       
 

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