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Antibody-Oligonucleotide All-in-One Conjugation Kit

Proven kits for ImmunoPCR, PLA, ECPA, and more.

No chromatography required!

The first referenced, easy-to-use kit to conjugate antibodies with oligos

The Antibody-Oligonucleotide All-in-One Conjugation Kit offers an innovative TurboLink™ catalyzed conjugation based on Chromalink™ technology to prepare antibody-oligonucleotide conjugates without chromatography. This novel kit includes buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation Kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

Download White Paper: Antibody-Oligonucleotide Conjugate Preparation

How it works
Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:

  1. Amine-modified, 20 to 60-mer oligonucleotide is modified using an excess of the Sulfo-S-4FB linker. This reactive NHS-ester incorporates a 4FB (aromatic aldehyde functional group, formylbenzamide) at the desired terminus of the oligonucleotide.
  2. Polyclonal or monoclonal antibody (100 g) is modified using the S-HyNic linker. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazino-nicotinamide) onto the antibody.
  3. The two modified biomolecules are mixed together in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds, followed by magnetic-affinity, solid phase purification.
  4. The antibody-oligonucleotide conjugate is ready for use in the most demanding and sensitive applications.

Advantages

  • Simple and Easy to Use – Requires only micropipette, microcentrifuge, and UV spectrophotometer
  • Automated Calculations – Calculator with fully integrated input/output provided
  • High Yield – 30–50% yield based on starting antibody
  • High Purity >95% purity without chromatographic purification
  • High Stability – Conjugates are stable for >1 year
       
 

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