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Linker Selection Guide

   
Instructions:

Chromalink™ technology requires two linkers to successfully conjugate 2 biomolecules to give you a quantifiable, controllable and stable result.  Determine how to link It with the following steps:
 
Step 1: Select type of conjugation from column A
Step 2: Select the 1st biomolecule you are working with from column B. Select the reactive group you are working with as well as additional options from column C.  The Solulink product you will need appears in column D.

Step 3: Select the 2nd biomolecule you are working with from column B.  The Solulink product you will need appears in column C. Select the reactive group you are working with as well as additional options from column C.  The Solulink product you will need appears in column D.

Step 4: Order the products referenced in steps 2 and 3 for a successful conjugation.
 
A Type of Conjugate  B- Type of Biomolecule CReactive Group   D Solulink Product
Antibody / Protein Ab If using an Amino (NH2) then use: Linker 1  S-HyNic [S-1002]
If using a Thiol (SH) then use: MHPH [S-1009]
Protein   If using an Amino (NH2) then use:

Linker 2

S-4FB [S-1004]
         
Protein / Oligo Protein   If using an Amino (NH2) then use: Linker 1 S-HyNic [S-1002]
If using a Thiol (SH) then use: MHPH [S-1009]
Oligo   If using a 3’ or 5’ Amino, then use: Linker 2   S-4FB [S-1004]
If using 4FB-phosphoramidite then use: S-4FB [S-1013]
         
Protein / Peptide Protein If using an Amino (NH2) then use: Linker 1 S-4FB [S-1004]
Peptide If using N-terminus, then use: Linker 2 BOC-HyNic [S-3003]
         
Oligo / Peptide Oligo If using 3’ or 5’ Amino, then use: Linker 1 S-4FB [S-1004] or sulfo-S-4FB [S-1008]
If using 3’ or 5’ Amino, then use: SS-S-4FB [S-1037-010] cleavable
If using 4FB-phosphoramidite, then use: S-4FB [S-1013]
Peptide If using N-terminus, then use: Linker 2 BOC-HyNic [S-3003]
If using C-terminus, then use:
         
Protein / NanoLink Beads Conjugation NanoLink Beads If using 4FB NanoLink Beads, then use: Linker 1   NanoLinkBeads [M-1001]  
Ab
Other protein
rPE, APC, perCP HRP, AlkPhos
If using an Amino (NH2), then use: Linker 2 S-HyNic [S-1002]
If using Thiol (SH), then use: MHPH [S-1009]
         
NanoLink Beads / Peptide Conjugation NanoLink Beads If using 4FB NanoLink Beads, then use: Linker 1 NanoLinkBeads [M-1001]
Peptide If using N-terminus, then use: Linker 2 BOC-HyNic [S-3003]
If using C-terminus, then use:
 

 

 

       
 

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