Epigenetic Publications

Formamide as a denaturant for bisulfite conversion of genomic DNA: Bisulfite sequencing of the GSTPi and RARbeta2 genes of 43 formalin-fixed paraffin-embedded prostate cancer specimens.

Zon G, Barker MA, Kaur P, Groshen S, Jones LW, Imam SA, Boyd VL.

Anal Biochem. 2009 Sep 15;392(2):117-25. doi: 10.1016/j.ab.2009.06.001. Epub 2009 Jun 6.

Preparation of genome-wide DNA fragment libraries using bisulfite in polyacrylamide gel electrophoresis slices with formamide denaturation and quality control for massively parallel sequencing by oligonucleotide ligation and detection.

Ranade SS, Chung CB, Zon G, Boyd VL.

Anal Biochem. 2009 Jul 15;390(2):126-35. doi: 10.1016/j.ab.2009.04.020. Epub 2009 Apr 18.

Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.

Boyd VL, Zon G.

Anal Biochem. 2008 Sep 1;380(1):13-20. doi: 10.1016/j.ab.2008.05.017. Epub 2008 May 20.

Methylation-dependent fragment separation: novel analysis of 5-methyl cytosine by capillary electrophoresis of amplified DNA using PCR incorporation of chemically modified dCTP.

Boyd VL, Moody KI, Karger AE, Livak KJ, Zon G, Burns JW.

Nucleosides Nucleotides Nucleic Acids. 2007;26(6-7):629-34.

Methylation-dependent fragment separation: direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA.

Boyd VL, Moody KI, Karger AE, Livak KJ, Zon G, Burns JW.

Anal Biochem. 2006 Jul 15;354(2):266-73. Epub 2006 May 6.

Bisulfite conversion of genomic DNA for methylation analysis: protocol simplification with higher recovery applicable to limited samples and increased throughput.

Boyd VL, Zon G.

Anal Biochem. 2004 Mar 15;326(2):278-80. No abstract available.

10 years ago
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