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You are at: All > Bioconjugation Reagents > Antibody Labeling

B-9007-105
ChromaLink™ Biotin Antibody Labeling Kit


ChromaLink™ Biotin Antibody Labeling Kit

Convenient kit boosts biotinylation reproducibility


The ChromaLink™ Biotin Antibody Labeling Kit provides everything you need for convenient, measuable, antibody or protein biotinylation. The kit contains ChromaLink™ Biotin, which incorporates a UV-traceable chromophore, based on  Chromalink™ technology, to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours (HABA), without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan (A280 and A354), you can quantify biotin incorporation and ensure reproducible production of consistent batches. A large scale kit is also available (labels 2 mg of antibody).

Workflow Diagram

Download White Paper: How to Biotinylate with Quantifiable Results

 

The ChromaLink™ Biotin Antibody Labeling Kit includes everything you need to biotinylate your antibody: buffers, reagents, spin columns, and an easy-to-follow protocol. With the ChromaLink™ Biotin Antibody Labeling Kit, you can biotinylate and purify up to 5 antibodies or proteins ranging in molecular weight from 20200 kDa from as little as 25 µg to as much as 1 mg in about 2.5 hours. The resulting biotinylated antibody is ready for binding to streptavidin. We recommend our NanoLink™ Streptavidin Magnetic Beads (Cat. No. M-1002-010) with the highest biotin-binding capacity available for assay development.

ChromaLink™ Biotin Antibody Labeling Kit Advantages

  • Reproducible Production  UV-traceable chromophore permits nondestructive, rapid A280/A354 quantification of incorporated biotin, enabling consistency and reproducibility

  • Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility

  • Scalable – Labels 25 µg to 1 mg of antibody

  • Convenient – Kit contains buffers, spin columns, and calculator to determine reaction quantities


ChromaLink™ Biotin Labeling Kit
Pierce EZ-Link Sulfo-NHS-Biotinylation Kit
Biotinylation Time
2.5 hours
2.5 hours
Quantification of Biotin
5 minute UV Scan = Free
3 hour HABA Assay = $140

 

ChromaLink™ Biotin Labeling Kit
Pierce EZ-Link Sulfo-NHS-Biotinylation Kit
Life Technologies APEX™ Biotin-XX Antibody Labeling Kit
Biotin Quantification
Yes
No
No
Complete Kit
Yes
No
No
Purification
Yes
No
No

 

 

 

 

Additional Information

 

 
  • Protocol
  • Calculators (Labeling and MSR)
  • Certificate of Analysis
  • MSDS

  • Citations

     


    1. Sweetwyne MT, Pallero MA, Lu A, Van Duyn Graham L, Murphy-Ullrich JE. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue Remodeling. American Journal of Pathology 2010;177(4):1710-24. Link
    2. Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945. Link
    3. Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027. Link
    4. Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767. Link
    5. Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108. Link

    Products and materials supplied by Solulink are for the purchaser's internal research use only. Internal research use only for scientific investigation performed at the purchaser's organization. Solulink material may not be used for any diagnostic, therapeutic or commercial use without a license from Solulink. Commercial purpose means any use for which you are compensated including any use in the manufacture of a product, information or data sale or any resale, repackaging or other transfer for consideration. Commercial licenses are available, please inquire. 

     

     
    Solulink ChromaLink™ Biotin Labeling Kit
    Pierce EZ-Link Sulfo-NHS-Biotinylation Kit
    Life Technologies APEX™ Biotin-XX Antibody Labeling Kit
    Biotin Quantification
    Yes
    No
    No
    Complete Kit
    Yes
    No
    No
    Purification
    Yes
    No
    No

     




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       Kit- labels 25 µg to 1 mg ($473.00)


     



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