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You are at : All > Nucleotides > Nucleotides by Structural Class > Alpha Phosphate Modified Nucleoside Triphosphates

Alpha Phosphate Modified Nucleoside Triphosphates

TriLink offers all four of the alpha thiol modified dNTPs, in addition to their RNA counterparts. The alpha borano dNTP series is also available.
 
Alpha thiol nucleotides were first introduced over 30 years ago and remain a valuable molecular biology tool. The foremost applications of these nucleotides are imparting nuclease resistance to an aptamer and introducing nicking sites in nick translation. 

Nick translation is one of the most common methods for introducing labeled nucleotides into a double stranded DNA molecule. It involves making small nicks in one strand of the DNA with DNAse and subsequently incorporating thio modified nucleotides by repairing them with DNA polymerase I. When alpha thio modified dNTPs are used in PCR, phosphorothioate-based sequencing is possible.

 

2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

Alpha Thiol dATP, 1-Thio-dATP


2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

Alpha Thiol dCTP, 1-Thio-dCTP


2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

Alpha Thiol dGTP, 1-Thio-dGTP


2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

Alpha Thiol dTTP, 1-Thio-dTTP


Adenosine-5'-O-(1-Thiotriphosphate)

Adenosine-5'-O-(1-Thiotriphosphate)

Alpha Thiol ATP, 1-Thio-ATP


Cytidine-5'-O-(1-Thiotriphosphate)

Cytidine-5'-O-(1-Thiotriphosphate)

Alpha Thiol CTP, 1-Thio-CTP


Guanosine-5'-O-(1-Thiotriphosphate)

Guanosine-5'-O-(1-Thiotriphosphate)

Alpha Thiol GTP, 1-Thio-GTP


Uridine-5'-O-(1-Thiotriphosphate)

Uridine-5'-O-(1-Thiotriphosphate)

Alpha Thiol UTP, 1-Thio-UTP


2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)

2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)

Alpha Thiol ddATP, 1-Thio-ddATP


2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)

2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)

Alpha Thiol ddCTP, 1-Thio-ddCTP


2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate)

2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate)

Alpha Thiol ddGTP, 1-Thio-ddGTP




3'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

3'-Deoxythymidine-5'-O-(1-Thiotriphosphate)

Alpha Thiol ddTTP, 1-Thio-ddTTP


3'-Azido-2',3'-dideoxythymidine-5'-O-(1-Thiotriphosphate)

3'-Azido-2',3'-dideoxythymidine-5'-O-(1-Thiotriphosphate)

Alpha Thiol-2',3'-Azido-ddTTP, 1-Thio-2',3'-Azido-ddTTP


2',3'-Dideoxyuridine-5'-O-(1-Thiotriphosphate)

2',3'-Dideoxyuridine-5'-O-(1-Thiotriphosphate)

Alpha Thiol ddUTP, 1-Thio-ddUTP


2'-Deoxyadenosine-5'-O-(1-Boranotriphosphate)

2'-Deoxyadenosine-5'-O-(1-Boranotriphosphate)

1-Borano-dATP


2'-Deoxycytidine-5'-O-(1-Boranotriphosphate)

2'-Deoxycytidine-5'-O-(1-Boranotriphosphate)

1-Borano-dCTP


2'-Deoxyguanosine-5'-O-(1-Boranotriphosphate)

2'-Deoxyguanosine-5'-O-(1-Boranotriphosphate)

1-Borano-dGTP


2'-Deoxythymidine-5'-O-(1-Boranotriphosphate)

2'-Deoxythymidine-5'-O-(1-Boranotriphosphate)

1-Borano-dTTP




 

 


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