Biotin Labeled Nucleoside Triphosphates
Biotin, a small molecule hapten that binds to the protein avidin, is perhaps the most widely used capture system. The affinity of biotin to avidin and streptavidin is extremely high, making it a very efficient molecular biology tool. While the binding of biotin to streptavidin is too strong to be easily reversed, the use of the biotin analog desthiobiotin allows for easy reversal of this interaction by washing the conjugate with large excesses of free biotin. The most common application is to bind biotin labeled nucleic acids to solid support labeled with avidin or streptavidin, in affinity capture/purification, hybrid capture, immunoassays, labeling and nucleic acid detection.
Our biotinylated nucleotides are modified in positions that will not affect the ability of the base to hybridize to its partner.