2' Fluoro and 2' O-Methyl NTPs are being utilized in an increasing number of applications in research and new drug development. Recently 2' Fluoro dATP was confirmed to be a universal tool for screening ATP-requiring enzymes in the investigation of pharmaceutically applicable kinases.
However, 2' Fluoro and 2' O-Methyl NTPs are most commonly enzymatically incorporated for improvement of in vivo stability in both DNA and RNA. They are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and nuclease stability while reducing immune response. 2' O-Me modifications in particular form very stable chimeric duplexes with standard RNA. 2' Fluoro and 2' O-Methyl NTPs can be incorporated as substrates for RNA polymerases, with T7 R&DNA™ polymerase and SP6 R&DNA™ polymerases from Epicentre providing the best results.