The most common application of sugar modified DNA triphosphates is chain termination
.TriLink offers the widest selection of 2′ modified 2′,3′ dideoxynucleoside triphosphates available, from post synthetically modifiable 2′ amino ddNTP for standard conjugations to 2′ azido ddNTP for click chemistry
Sugar modified RNA nucleotides, on the other hand are used to impart nuclease resistance
to RNA. The molecule retains the A-form duplex structure characteristic of RNA, but modification of the 2′ hydroxyl, which is often crucial to either the binding or the activity of the enzyme, will offer a level of protection against nuclease degradation and other enzymatic events. The most popular sugar modified NTPs are the 2′ O-methyl modified NTPs, followed by the 2′ fluoro modified NTPs. Both of these modifications will increase the melting temperature of a duplex by 1 to 3°C per insertion depending on which modification is used, the base and position.