mRNA Length: 996 nucleotides
Concentration: 1.0 mg/mL
Buffer: 1 mM Sodium Citrate, pH 6.4
Agarose Gel Mobility; Pass
Cyanine 5 EGFP mRNA is designed for the analysis of mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. When labeled with cyanine 5, EGFP mRNA can be directly visualized. Cyanine 5 EGFP mRNA is an ideal molecule to determine mRNA delivery and localization independent of translation.
Cyanine 5 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 650 nm and 670 nm respectively. TriLink’s CleanCap Cyanine 5 EGFP mRNA is transcribed with Cyanine 5-UTP:5-Methoxy-UTP at a ratio of 1:3. Substitution in this ratio results in mRNA that is easily visualized and can still be translated in cell culture. Translation efficiency correlates inversely with Cyanine 5-UTP substitution.
This mRNA is capped using CleanCap®, TriLink's proprietary co-transcriptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
Certificate(s) of Analysis
L-7701 is a replacement for L-6402.
Sold for research use only under license
from Life Technologies.
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