mRNA Length: 1,921 nucleotides
Concentration: 1.0 mg/mL
Buffer: 1 mM Sodium Citrate, pH 6.4
Agarose Gel Mobility; Pass
Concentration ± 6%; Pass
CleanCap® Cyanine 5 FLuc mRNA is designed for the analysis of mRNA delivery and translation efficiency. The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin. When labeled with cyanine 5, FLuc mRNA can be directly visualized. CleanCap™ Cyanine 5 FLuc mRNA is an ideal molecule to determine mRNA delivery and localization independent of translation.
Cyanine 5 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 650 nm and 670 nm respectively. TriLink’s CleanCap® Cyanine 5 FLuc mRNA is transcribed with Cyanine 5-UTP:5-Methoxy-UTP at a ratio of 1:3. Substitution in this ratio results in mRNA that is easily visualized and can still be translated in cell culture. Translation efficiency correlates inversely with Cyanine 5-UTP substitution.
This mRNA is capped using CleanCap®, TriLink's proprietary co-transcriptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
Certificate(s) of Analysis
L-7702 is a replacement for L-6401.
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