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Sulfo-S-4FB Crosslinker (Water Soluble)

Sulfo-S-4FB Crosslinker (Water Soluble)

The S-4FB (N-succinimidyl-4-formylbenzamide) heterobifunctional crosslinker is fundamental to Chromalink®technology. Sulfo-S-4FB is a water soluble analog of S-4FB. S-4FB analog reacts with primary amines on proteins (amino group of lysine) or amino-modified oligonucleotides or surfaces, introducing a 4FB (4-formylbenzamide) linker that forms stable covalent conjugates with biomolecules possessing HyNic (6-hydrazinonicotinamide) incorporated linkers. 4FB groups incorporated on biomolecules and surfaces are extremely stable and have been known to maintain their reactivity for >18 months. Sulfo-S-4FB is recommended for incorporating 4FB linkers on amino-surfaces or biomolecules.  This linker is particularly useful for biomolecules or surfaces that are sensitive to organic solvents such as DMF or DMSO.


Chromalink® technology is based on the use of two complementary heterobifunctional linkers:  

  • S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
  • S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
  • HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

TriLink Advantages

  • Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
  • Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
  • Stability – 10 times more stable than any other conjugation linker
  • Specificity – Two-linker method avoids homoconjugate formation
  • Efficient – >80% efficient linker-biomolecule conjugations
  • Robust – The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0

Additional Information


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