MHPH (Maleimide HyNic) Crosslinker
MHPH (Maleimide HyNic) is a maleimide that converts thiols on biomolecules and surfaces to HyNic linker molecules. The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Chromalink® technology. MHPH converts cysteine or thiol groups to HyNic linkers in a single step. The S-HyNic analog reacts with primary thiols on proteins (cysteine residues) introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers. The advantages of the MHPH-4FB linker system include reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process.
Chromalink® technology is based on the use of two complementary heterobifunctional linkers:
- S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
- S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
- HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.
The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.
- Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
- Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
- Stability – 10 times more stable than any other conjugation linker
- Specificity – Two-linker method avoids homoconjugate formation
- Efficient – >80% efficient linker-biomolecule conjugations
- Robust - The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0
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