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MHPH (Maleimide HyNic) Crosslinker

MHPH (Maleimide HyNic) Crosslinker

 MHPH (Maleimide HyNic) is a maleimide that converts thiols on biomolecules and surfaces to HyNic linker molecules. The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Chromalink® technology. MHPH converts cysteine or thiol groups to HyNic linkers in a single step. The S-HyNic analog reacts with primary thiols on proteins (cysteine residues) introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers. The advantages of the MHPH-4FB linker system include reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process.


Chromalink® technology is based on the use of two complementary heterobifunctional linkers:  

  • S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
  • S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
  • HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

TriLink Advantages

  • Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
  • Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
  • Stability – 10 times more stable than any other conjugation linker
  • Specificity – Two-linker method avoids homoconjugate formation
  • Efficient – >80% efficient linker-biomolecule conjugations
  • Robust - The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0


Additional Information


  1. Shen ZT, Brehm MA, Daniels KA, Sigalov AB, Selin LK, Welsh RM, Stern LJ. BI-specific MHC heterodimers for characterization of cross-reactive T cells. Journal of Biological Chemistry 2010;285(43):33144-53. Link 
  2. Kwong GA, Radu CG, Hwang K, Shu CJ, Ma C, Koya RC, Comin-Anduix B, Hadrup SR, Bailey RC, Witte ON. Modular nucleic acid assembled p/MHC microarrays for multiplexed sorting of antigen-specific T cells. Journal of the American Chemical Society 2009;131(28):9695-9703. Link 
  3. DeRouchey J, Schmidt C, Walker GF, Koch C, Plank C, Wagner E, Rädler JO. Monomolecular Assembly of siRNA and Poly (ethylene glycol)- Peptide Copolymers. Biomacromolecules 2008;9(2):724-732. <NO LINK>
  4. Levashova Z, Backer J, Backer M, Blankenberg F. Direct labeling of single-chain VEGF (sc-VEGF) with Tc99m.  In: Society of Nuclear Medicine Annual Meeting Abstracts.  Soc Nuclear Med; 2007 p. 181P. Link  

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

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