3′‐O‐(4‐oxotetradec‐1‐yl) protected Phosphodiester DNA is a thermolabile protecting group which can block annealing between a Hot Start PCR Primer (known as CleanAmp Primers) and the target. 3' OXT is also known as 3'-C10-OXP. Once deprotected, it performs as a standard phophodiester backbone. CleanAmp® Primers are available in two forms that differ in the rate of thermal activation.
Per Base Pricing
5.0 µmole $1,400
10 µmole $2,600
15 µmole $3,800
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CleanAmp Precision Primers contain two modifications on the 3' end, consequently, activating more slowly than CleanAmp Turbo Primers, which is beneficial for different applications and PCR needs, such as:
• Standard cycling
• One-step reverse-transcription PCR
• Improving specificity and limit of detection
• Providing the greatest reduction in mis-priming/primer dimer formation
CleanAmp Turbo Primers contain a single modification on the 3' end, resulting in a slower activation than CleanAmp Precision Primers, which is beneficial for different applications and PCR needs, such as:
• Fast cycling
• Mulitplex PCR
• Improving amplicon yield
• Reducing mis-priming/primer dimer formation
CleanAmp Primers can be cartridge purified. However, for those applications where PCR performance is of the utmost importance, we can RP-HPLC purify your CleanAmp® Primers.
CleanAmp Primer Product Literature
CleanAmp® is a trademark of TriLink BioTechnologies, LLC. CleanAmp Products, technology and their use may be covered by one or more patents or pending patent applications and provided under a Research License Agreement from TriLink BioTechnologies, LLC.