Extinction Coefficient: 13,120 Lmol-1cm-1 at 274 nm
Molecular Weight: 500.2 g/mol (free acid)
Molecular Formula: C9H15N2O14P3S (free acid)
Salt Form: Lithium
Purity Specification: ≥95% by AX-HPLC
Typically shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL
Certificate(s) of Analysis
2-Thiouridine-5'-Triphosphate (2-Thio-UTP) is a substrate for several DNA polymerases and will substitute for UTP (Nakayama et al.). 2-Thio-UTP is an inhibitor of norovirus RNA dependent RNA polymerase (Belliot et al.). RNAs prepared from 2-Thio-UTP is less immunogenic as compared to unmodified RNA. 2-Thio-U modified mRNA shows no activation of RNA-dependent protein kinase (Anderson et al.), significantly reduced levels of 2'-5'-oligoadenylate synthetase activation (Anderson et al.), reduced levels of retinoic acid-inducible protein I (Hornung et al.) and suppression of activation of Toll-like receptors (Kariko et al.). However, 2-Thio-U modified mRNAs show poor translation efficiency in cell-free systems and in cultured cells (Kariko et al., Zimmer et al.). 2-Thio-UTP is a competitive inhibitor of CTP synthetase (Scheit et al.) and a potent and selective agonist of P2Y2 human receptor (El-Tayeb, et al., Jacobson et al., Ko et al., Govindan et al.).