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You are at: All > Nucleotides > Nucleotides by Structural Class > Sugar Modified Nucleoside Triphosphates

N-4002
2',3'-Dideoxyguanosine-5'-Triphosphate
ddGTP

2',3'-Dideoxyguanosine-5'-Triphosphate

Extinction Coefficient: 13,600 Lmol-1cm-1 at 252 nm
Molecular Weight: 491.1 g/mol (free acid)
Molecular Formula: C10H16N5O12P3 (free acid)
Salt Form: Lithium
Purity Specification: ≥95% by AX-HPLC

Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL


 
Certificate(s) of Analysis
N4002-T1ZM01A-5

2',3'-Dideoxyguanosine-5'-Triphosphate (ddGTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddGTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
 
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."

Michal Legiewicz Research Scientist



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Reference(s)
Smith TM, et al. Identifying Initiation and Elongation Inhibitors of Dengue Virus RNA Polymerase in a High-Throughput Lead-Finding Campaign. J Biomol Screen. 2014 Sep 24.
Mizrahi RA, Schirle NT, Beal PA. Potent and Selective Inhibition of A-to-I RNA Editing with 2'-O-Methyl/Locked Nucleic Acid-Containing Antisense Oligoribonucleotides. ACS Chem Biol. 2013 Feb 21. doi:10.1021/cb300692k.
Vooradi V, Romano L. Effect of N-2-Acetylaminofluorene and 2-Aminofluorene Adducts on DNA binding and Synthesis by Yeast DNA Polymerase n. (2009) Biochemistry. (48): 4208-4216.
Sahu S, LaBean TH, Reif JH. A DNA Nanotransport Device Powered by Polymerase phi29. (2008) Nano Letters (8)(11): 3870-3878.
Heck J, et al. Effects of Mutagenic and Chain-Terminating Nucleotide Analogs on Enzymes Isolated from Hepatitis C Virus Strains of Various Genotypes. Antimicrobial Agents and Chemotherapy.
Liu X, et al. 3'-Azido-2',3'-dideoxynucleoside 5'-triphosphates inhibit telomerase activity in vitro, and the corresponding nucleosides cause telomere shortening in human HL60 cells, Nucleic Acid Research.
Imbert I, Guillemot J, Bourhis J, et al. A second non-canonical RNA-dependent polymerase in SARS Coronavirus. (2006) EMBO Journal, (25): 4933-4942.
Tang K, Oeth P, Kammerer S, et al. Mining Disease Susceptibility Genes through SNP Analyses and Expression Profiling Using MALDI-TOF Mass Spectrometry. (2004) J. Proteome Res. (3): 218-227.
Roettger MP, Fiala KA, Sompalli S, Dong Y, Suo Z. Pre-steady-state kinetic studies of the fidelity of human DNA polymerase mu. (2004) Biochemistry, (43)(43): 13827-13838.


 

 


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