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You are at: All > Nucleotides > Nucleotides by Structural Class > Sugar Modified Nucleoside Triphosphates



Extinction Coefficient: 13,600 Lmol-1cm-1 at 252 nm
Molecular Weight: 491.1 g/mol (free acid)
Molecular Formula: C10H16N5O12P3 (free acid)
Salt Form: Lithium
Purity Specification: ≥95% by AX-HPLC

Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL

Certificate(s) of Analysis

2',3'-Dideoxyguanosine-5'-Triphosphate (ddGTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddGTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."

Michal Legiewicz Research Scientist

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