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You are at: All > Nucleotides > Nucleotides by Structural Class > Sugar Modified Nucleoside Triphosphates



Extinction Coefficient: 10,100 Lmol-1cm-1 at 262 nm
Molecular Weight: 452.1 g/mol (free acid)
Molecular Formula: C9H15N2O13P3 (free acid)
Salt Form: Lithium
Purity Specification: ≥90% by AX-HPLC

Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL


Certificate(s) of Analysis

2',3'-Dideoxyuridine-5'-Triphosphate (ddUTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddUTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."

Michal Legiewicz Research Scientist

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Smith TM, et al. Identifying Initiation and Elongation Inhibitors of Dengue Virus RNA Polymerase in a High-Throughput Lead-Finding Campaign. J Biomol Screen. 2014 Sep 24.
Boyle NA, et al. Synthesis of 2',3'-dideoxynucleoside 5'-alpha-P-borano-beta,gamma-(difluoromethylene)triphosphates and their inhibition of HIV-1 reverse transcriptase. (2005) Med. Chem., (48)(7): 2695-2700.
Hao Z, Cooney DA, Farquhar D, Perno CF, et al. Potent DNA chain termination activity and selective inhibition of human immunodeficiency virus reverse transcriptase by 2',3'-dideoxyuridine-5'-triphosphate. (1990) Mol. Pharmacol, (37): 157-163.
Lin TS, et al. Synthesis and antiviral activity of various 3'-azido, 3'-amino, 2',3'-unsaturated, and 2',3'-dideoxy analogues of pyrimidine deoxyribonucleosides against retroviruses. (1987) J. Med. Chem.,(30): 440–444.



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