My Account | Shopping Cart | Contact  
Trilink Biotech Logo

Oligonucleotides | mRNA & Long RNA | Nucleotides | CleanCap™ | CleanTag™ | CleanAmp™ | Custom Chemistry | GMP

OligoBuilder® | Nucleotides List | Stocked mRNA List | DNA Synthesis Reagents List | CleanAmp™ Products List


You are at: All > Nucleotides > Nucleotides by Structural Class > Sugar Modified Nucleoside Triphosphates

N-4004
2',3'-Dideoxythymidine-5'-Triphosphate
ddTTP

2',3'-Dideoxythymidine-5'-Triphosphate

Extinction Coefficient: 9,650 Lmol-1cm-1 at 267 nm
Molecular Weight: 466.1 g/mol (free acid)
Molecular Formula: C10H17N2O13P3 (free acid)
Salt Form: Lithium
Purity Specification: ≥95% by AX-HPLC

Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL


MSDS

Certificate(s) of Analysis
2',3'-Dideoxythymidine-5'-Triphosphate (ddTTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
 
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."

Michal Legiewicz Research Scientist



Email a Friend
Product Inquiry
    
Ready To Order?

Please select a size :
   1 µmole ($32.00)
   5 µmoles ($134.00)
   10 µmoles ($243.00)


 



Related Products :

Related Categories :


Reference(s)
Mizrahi RA, Schirle NT, Beal PA. Potent and Selective Inhibition of A-to-I RNA Editing with 2'-O-Methyl/Locked Nucleic Acid-Containing Antisense Oligoribonucleotides. ACS Chem Biol. 2013 Feb 21. doi:10.1021/cb300692k.
Brown J, Suo Z. Elucidating Mechanism of DNA Polyemerization Catalyzed by Sulfolobue solfataricus P2 DNA Polymerase B1. (2009) Biochemistry, (48):7502-7511.
Vooradi V, Romano L. Effect of N-2-Acetylaminofluorene and 2-Aminofluorene Adducts on DNA binding and Synthesis by Yeast DNA Polymerase n. (2009) Biochemistry. (48): 4208-4216.
Sahu S, LaBean TH, Reif JH. A DNA Nanotransport Device Powered by Polymerase phi29. (2008) Nano Letters (8)(11): 3870-3878.
Tang K, Oeth P, Kammerer S, et al. Mining Disease Susceptibility Genes through SNP Analyses and Expression Profiling Using MALDI-TOF Mass Spectrometry. (2004) J. Proteome Res. (3): 218-227.
Roettger MP, Fiala KA, Sompalli S, Dong Y, Suo Z. Pre-steady-state kinetic studies of the fidelity of human DNA polymerase mu. (2004) Biochemistry, (43)(43): 13827-13838.
Lin TS, et al. Synthesis and antiviral activity of various 3'-azido, 3'-amino, 2',3'-unsaturated, and 2',3'-dideoxy analogues of pyrimidine deoxyribonucleosides against retroviruses. (1987) J. Med. Chem.,(30): 440–444.


 

 


  Twitter Twitter Facebook Facebook Linked In Linked In RSS RSS Google RSS  
© 2018 TriLink BioTechnologies, LLC | All Rights Reserved