Extinction Coefficient: 9,650 Lmol-1cm-1 at 267 nm
Molecular Weight: 466.1 g/mol (free acid)
Molecular Formula: C10H17N2O13P3 (free acid)
Salt Form: Lithium
Purity Specification: ≥95% by AX-HPLC
Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL
Certificate(s) of Analysis
2',3'-Dideoxythymidine-5'-Triphosphate (ddTTP) is a sugar modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is useful in a variety of biotechnology applications. ddTTP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations.
"I use ddNTPs from TriLink for sequencing using DNA and RNA as templates and it always gives me good results."