Extinction Coefficient: 21,000 Lmol-1cm-1 at 254 nm
Molecular Weight: 803.4 g/mol (free acid)
Molecular Formula: C21H30N10O18P3 (free acid)
Salt Form: NH4+
Purity Specification: ≥90% by AX-HPLC
Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL
Eukaryotic and viral messenger RNA (mRNA
) has a monomethylated cap structure, m7G(5')ppp(5')G or mCAP, at the 5’-terminus. The cap analog plays an important role in cellular processes, most notably the initiation of mRNA translation. The cap interacts with different binding proteins, initiation factors and elements of ribosomes.
mCAP was the first cap analog to be introduced to the molecular biology toolbox. Half of the time, mCAP inserts in the correct orientation to enhance translation. The other 50% of molecules are not substrates for efficient translation, reducing the specific activity of the transcript. More recently, Anti Reverse Cap Analog (ARCA)
was introduced. ARCA can only insert in the proper orientation, resulting in mRNAs that are translated twice as efficiently as those initiated with mCAP.
mCAP is incorporated in a transcription by including a mixture of the cap analog and GTP (usually at a 4:1 ratio). Approximately 80% of synthesized mRNA
will possess a 5’ cap, while the remaining 20% will possess a 5’ triphosphate.