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You are at: All > Oligonucleotides > Custom Oligonucleotide Components > Custom Oligonucleotide Components by Type > Terminal Phosphates

5' Phosphate

 



5' Phosphate

Oligonucleotide Modification Price
HPLC Purified
5.0 µmole $236
10 µmole $315
15 µmole $394
Other Purification
5.0 µmole $173
10 µmole $231
15 µmole $289

5' Phosphorylation requires additional processing in order to ensure high quality and yield. Phosphates can be placed on the 3' or 5' termini of oligonucleotides. A 3' terminal phosphate can be used to block elongation although other modifications, such as 3' Propyl or a 2',3'-Dideoxy modified base, are more effective terminators. A 5' terminal phosphate is needed for enzymatic ligation of two oligonucleotides to form longer oligonucleotides.



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Reference(s)
Clark TA, Lu X, Luong K, Dai Q, Boitano M, Turner SW, He C, Korlach J. Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation. BMC Biol. 2013 Jan 22;11(1):4.
Jahic H, Liu CF, Thresher J, Livchak S, Wang H, Ehmann DE. The kinetic mechanism of S. pneumoniae DNA ligase and inhibition by adenosine-based antibacterial compounds. Biochem Pharmacol. 2012 Jun 25. doi: 10.1016/j.bcp.2012.06.017.
Gu GJ, et. al. Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol. 2012 Jun 2. doi:10.1016/j.nbt.2012.05.005.
Piecyk K, Davis RE, Jankowska-Anyszka M. 5'-Terminal chemical capping of spliced leader RNAs. Tetrahedron Letters. 2012 Jul 7. doi:10.1016/j.tetlet.2012.06.127.
Shapiro AB, Gao N, Hajec L, McKinney DC. Time-dependent, reversible, oxaborole inhibition of Escherichia coli leucyl-tRNA synthetase measured with a continuous fluorescence assay. Anal Biochem. 2012 Sep 10;431(1):48-53. doi: 10.1016/j.ab.2012.08.024
Duderstadt KE, Chuang K, Berger JM. DNA stretching by bacterial initiators promotes replication origin opening. (2011) Nature. doi: 10.1038/nature10455.


 

 


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