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You are at: All > Oligonucleotides > Custom Oligonucleotide Components > Custom Oligonucleotide Components by Type > Terminal Phosphates

3' Phosphate

 



3' Phosphate

Oligonucleotide Modification Price
5.0 µmole $252
10 µmole $336
15 µmole $420

Phosphates can be placed on the 3' or 5' termini of oligonucleotides. A 3' terminal phosphate can be used to block elongation although other modifications, such as 3' Propyl or a 2',3'-Dideoxy modified base, are more effective terminators. A 5' terminal phosphate is needed for enzymatic ligation of two oligonucleotides to form longer oligonucleotides.



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Reference(s)
Murakami T, Sumaoka J, Komiyama M. Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification. Nucleic Acids Res. 2012 Feb;40(3):e22.
Duderstadt KE, Chuang K, Berger JM. DNA stretching by bacterial initiators promotes replication origin opening. (2011) Nature. doi: 10.1038/nature10455.


 

 


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