Per Base Pricing
5.0 µmole $9.00
10 µmole $12.00
15 µmole $15.00
|Single RP-HPLC & RP-Cartridge
|PAGE followed by RP-HPLC
|5.0 μmole scale
||3.33 - 8.33
|10 μmole scale
||6.66 - 16.6
|15 μmole scale
||10.0 - 25.0
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*Expected yields are estimates for HPLC purified (~90%) unmodified oligonucleotides. Yield and purity differences can be caused by many factors, such as sequence and length. If you require a specific yield, please let us know when you place your order or request a quote.
The modification of oligonucleotides by exchanging a non-bridging oxygen on the phosphate backbone to form a phosphorothioate (PS) linkage has a long history. Initially introduced and explored by Prof. Fritz Eckstein mainly with modified nucleoside triphosphates in the 1970’s, the most significant application was described in the early 1980’s by a team from the NCI and NIH. They demonstrated the use of a phosphorothioate linkage to protect antisense oligonucleotides from nuclease degradation, prolonging it’s lifetime in serum.
To this day, the main application of phosphorothioate linkages in an oligo is to protect the oligo from nuclease degradation. It is the least expensive option and is fairly effective, although nuclease resistance is often further enhanced when combined with a 2’-OMe (2' O-Methyl ) modification
. Nuclease stability is essential for antisense and RNAi applications where oligos are exposed to exonucleases and endonucleases within cells. Phosphorothioates have become the most popular modification used in antisense oligonucleotides.
One drawback to adding phosphorothioate linkages is that it does destabilize duplexes, reducing the Tm (melting temperature, the temperature at which exactly one half the single strands of a duplex are hybridized) by 1-3° C per addition. This can also be largely overcome by the addition of 2’ OMe modifications which increases Tm by a similar amount. However, the portion of the oligo so modified will have properties more similar to RNA than to DNA.
TriLink is proud to be one of the leaders in custom phosphorothioate oligo (S-Oligo, thioate) synthesis. Our high purity standards are crucial for the synthesis of modified oligos without the cytotoxic issues that plagued PS oligonucleotides for decades. You can depend on TriLink to deliver the quality you need to succeed.