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You are at: All > Oligonucleotides > Custom Oligonucleotide Components > Custom Oligonucleotide Components by Type > Backbones (Purification Options & Expected Yields)

2' O-Methyl RNA (Phosphodiester & Phosphorothioate)
2' OMe

2' O-Methyl RNA (Phosphodiester & Phosphorothioate)

Per Base Pricing
0.2 µmole $12.00
1.0 µmole $16.00
5.0 µmole $45.00
10 µmole $60.00
15 µmole $75.00


Purification Method0.2 µmole1.0 µmole
Double RP-HPLC$100$150               
Single RP-HPLC & RP-Cartridge$50$75
AX-HPLC$75$125
RP-Cartridge$20$20
PAGE$75$125
PAGE followed by RP-HPLC$125$200
 
Purification Method5.0 µmole10 µmole15 µmole
Double RP-HPLC$275$325$375
Single RP-HPLC & RP-Cartridge$225$275$325
AX-HPLC$275$375$425
RP-CartridgeN/AN/AN/A
PAGE$500$750$1000
PAGE followed by RP-HPLC$725$1025$1325


Expected Yields*OD260 unitsapprox. mg
0.2 μmole scale5 - 150.15 - 0.50
1.0 μmole scale20 - 600.66 - 2.00
5.0 μmole scale100-2503.33 - 8.33
10 μmole scale200-5006.66 - 16.6
15 μmole scale300-75010.0 - 25.0

*Expected yields are estimates for HPLC purified (~90%) unmodified oligonucleotides. Yield and purity differences can be caused by many factors, such as sequence and length. If you require a specific yield, please let us know when you place your order or request a quote.

The 2′ O-Methyl oligo modification is best characterized as a RNA analog that offers stability against general base hydrolysis and nucleases, as well as increased Tm of duplexes by 1 - 4°C per addition.

The 2′ O Methyl oligonucleotide modification was introduced to the antisense oligo field long ago fairly simultaneously by research teams from Japan, Europe and the US. 2′ O-Methyl, a naturally occurring analog with a long history in the literature, is viewed as the perfect RNA analog because of its stability. The only real disadvantage with 2′ O Methyl oligo insertions is that they are not recognized by many enzymes, thereby limiting utility slightly. 2′ O-Methyl modifications are generally used in conjunction with DNA and are usually considered a solution for nuclease stability issues or the duplex stability of DNA molecules. However, it should be noted that the configuration of a 2′ O Methyl oligonucleotide is in the A-form like RNA, and not the B-form like DNA. It only takes a couple of these modifications in a row to effect the transition from one form to the other.

Another key application is the stabilization of RNA molecules either from nuclease activity in applications where the function of the RNA itself is not required, and is used merely as a duplex, or for higher order structure, such as in an aptamer.



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Reference(s)
Alidori S, Asqiriba K, Londero P, Bergkvist M, Leona M, Scheinberg DA, McDevitt MR. Deploying RNA and DNA with Functionalized Carbon Nanotubes. J. Phys. Chem. 2013 Feb 21. doi:10.1021/jp312416d
Hernandez FJ, Stockdale KR, Huang L, Horswill AR, Behlke MA, McNamara JO. Degradation of Nuclease-Stabilized RNA Oligonucleotides in Mycoplasma-Contaminated Cell Culture Media. Nucleic Acid Ther. 2012 Jan 9. doi: 10.1089/nat.2011.0316.
Leygue E, Guo J. Increasing the relative expression of endogenous non-coding Steroid Receptor RNA Activator (SRA) in human breast cancer cells using modified oligonucleotides, Nucleic Acid Research, May 2009, Pubmed. ID: 19483093).
Lahoud G, Arar K, Hou YM, Gamper H. RecA-mediated strand invasion of DNA by oligonucleotides substituted with 2-aminoadenine and 2-thiothymine. Nucleic Acids Res. 2008 Dec;36(21):6806-15.


 

 


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