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You are at: All > Oligonucleotides > Custom Oligonucleotide Components > Custom Oligonucleotide Components by Type > Backbones (Purification Options & Expected Yields)

2' Fluoro RNA
2' F RNA

2' Fluoro RNA

Per Base Pyrimidine Pricing (C/U)
5.0 µmole Inquire*
10 µmole Inquire*
15 µmole Inquire*
*2' Fluoro pricing at selected scales is based on the number of incorporations in your oligonucleotide.

Per Base Purine Pricing (A/G)
5.0 µmole $175.00
10 µmole $250.00
15 µmole $350.00

Per base pricing discounted for multiple incorporations. Minimum pricing requirements may apply. Please request a quote for exact pricing or contact us to speak with a customer service representative.

Purification Methods and Expected Yields
2' Fluoro RNA oligonucleotides can often be purified using the same methods as standard DNA oligonucleotides. However, this depends on the design of the entire molecule. Yields are extremely variable, affected by modifications and sequence. Please contact us for information regarding purification methods and expected yields for your specific construct.
 

The main applications of this modification are ribozyme development - to increase Tm, aptamer selection, and nuclease resistance. A substitution of 2’-fluoro for 2’-hydroxyl group in a RNA monomer does not substantially change the conformation of sugar ring puckering and other structural parameters thus making 2’-deoxy-2’-fluoro RNA oligonucleotide analogs (FRNA) a virtual mimic of natural RNAs. FRNA and RNA have many similar physical properties which allow FRNAs to form stable duplexes with RNA targets. The stability of FRNA-RNA duplexes is even higher than that for normal RNA-RNA duplexes (about 1-2oC per each substitution ). With DNA targets there is a moderate increase of Tm for DNA-FRNA duplexes (up to 1oC per substitution) compared to normal DNA-DNA duplexes.   Oligonucleotides containing 2’ fluoros have been reported to increase DNA-DNA Tm by 1.3°C per insertion.

The absence of the 2’-hydroxyl group makes the internucleotide linkages in oligonucleotides with 2’-deoxy-2’-fluoro much more stable to a chemical hydrolysis at ahigh pH compared to internucleotide linkages in unmodified RNA oligonucleotides. The increased chemical stability of 2’-deoxy-2’-fluoro RNA oligonucleotide analogs is accompanied by high resistance of 2’-deoxy-2’-fluoro linkages to ribonucleases and, consequently, leads to a longer survival time of FRNAs in biological environment (cell cultures, plasma, animals, etc.).

The biological activity of siRNAs constructed from 2’-deoxy-2’-fluoro units in cell culture systems and in vivo is similar or higher compared to activity of unmodified RNAs. Notably, siRNAs containing 2’-deoxy-2’-fluoro units have lower level of activation of immune response compared to unmodified siRNAs. However, duplexes of FRNA and RNA do not support RNAse H activity and therefore the use of FRNA oligonucleotides is questionable for antisense applications.

In addition, 2’-deoxy-2’-fluoro RNA analogs have been used in the construction and studies of ribozymes and oligonucleotide aptamers with enhanced affinity.




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Reference(s)
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