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You are at: All > Oligonucleotides > Custom Oligonucleotide Components > Custom Oligonucleotide Components by Type > Purification Methods

Desalt



Desalt

For some applications purification is not required. In these cases we will simply precipitate the oligonucleotide to desalt the product and remove any remaining organic compounds. For a standard unpurified oligonucleotide we expect ~70% purity, however we cannot guarantee purity for desalt only products.
 



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Reference(s)
York KT, Smith RC, Yang R, Melnyk PC, Wiley MM, Turk CM, Ronaghi M, Gunderson KL, Steemers, FJ. Highly Parallel Oligonucleotide Purification and Functionalization Using Reversible Chemistry. Nucleic Acids Res. 2012 Jan;40(1):e4.
Wilson TJ, Crystal MA, Rohrbaugh MC, Sokolowsky KP, Gindt YM. Evidence from Thermodynamics that DNA Photolyase Recognizes a Solvent Exposed CPD Lesion. (2011) J Phys Chem B. doi: 10.1021/jp208129a.
Banerjee S, Bhowmik D, Verma PK, Mitra RK, Siddhanto A, Basu G, Pal SK. Ultrafast Spectroscopic Study on Caffeine Mediated Dissociation of Mutagenic Ethidium from Synthetic DNA and Various Cell Nuclei. (2011) J Phys Chem B. doi: 10.1021/jp206300x


 

 


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