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Antibody-Oligonucleotide All-in-One Conjugation Kit

Antibody-Oligonucleotide All-in-One Conjugation Kit

No chromatography required!  

The Application-Proven kit to conjugate antibodies with oligos just got even better.

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Download the White Paper

The Antibody-Oligonucleotide All-in-One Conjugation Kit offers an innovative TurboLink™ catalyzed conjugation based on Chromalink™ technology to prepare antibody-oligonucleotide conjugates without chromatography. This novel kit includes everything you need —buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation Kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).

Download White Paper: Antibody-Oligonucleotide Conjugate Preparation and Applications

Workflow Diagram
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How it works
Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:  

  1. Amine-modified, 20 to 60-mer oligonucleotide is modified using an excess of the Sulfo-S-4FB linker. This reactive NHS-ester incorporates a 4FB (aromatic aldehyde functional group, formylbenzamide) at the desired terminus of the oligonucleotide.
  2. Polyclonal or monoclonal antibody (100 μg) is modified using the S-HyNic linker. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazino-nicotinamide) onto the antibody.
  3. The two modified biomolecules are mixed together in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds, followed by magnetic-affinity, solid phase purification.
  4. The antibody-oligonucleotide conjugate is ready for use in the most demanding and sensitive applications.



  • Simple and Easy to Use – Requires only micropipette, microcentrifuge, and UV spectrophotometer
  • Automated Calculations – Calculator with fully integrated input/output provided
  • High Yield – 30–50% yield based on starting antibody
  • High Purity –  >95% purity without chromatographic purification
  • High Stability – Conjugates are stable for >1 year

 Additional Information


  1. Noriyuki K.Kazuhiro K., Shinobu S., Yukitaka Y., Fusanori Y., Shingo M., Eisuke M. and Junichi E. Soluble heparin-binding EGF-like growth factor (HB-EGF) detected by a newly developed immuno-PCR method is a clear-cut serological biomarker for ovarian cancer. American Journal of Transitional Research, 2012, 4, 415-421.
  2. S. Fredriksson, M. Gullberg, J. Jarvius1, C. Olsson, K.Pietras, S.M Gustafsdottir, A. Ostman and U. Landegren, Protein detection using proximity-dependent DNA ligation assays, Nature Biotechnology 2002, 20, 473-477.
  3. Jiaming H., Tanyu W., Joonyul K., Curtis S., and Christopher E., Journal of the American Chemical Society, 2012, 134, 7066-7072.
  4. Schlingemann J, Leijon M, Yacoub A, Schlingemann H, Zohari S, Matyi-Tóth A, Kiss I, Holmquist G, Nordengrahn A, Landegren U. Novel means of viral antigen identification: Improved detection of avian influenza viruses by proximity ligation. Journal of virological methods 2010;163(1):116-122. Link
  5. Kamali-Moghaddam M, Pettersson E, Wu D, Englund H, Darmanis S, Lord A, Tavoosidana G, Sehlin D, Gustafsdottir S, Nilsson LNG. Sensitive detection of A-beta protofibrils by proximity ligation- relevance for Alzheimer's disease. BMC Neuroscience 2010;11(1):124. Link 
  6. Farrar D, Rai S, Chernukhin I, Jagodic M, Ito Y, Yammine S, Ohlsson R, Murrell A, Klenova E. Mutational analysis of the poly (ADP-ribosyl) ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly (ADP-ribosyl) ation. Molecular and cellular biology 2010;30(5):1199. Link
  7. Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vänelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U. Sensitive plasma protein analysis by microparticle-based proximity ligation assays. Molecular & Cellular Proteomics 2010;9(2):327. Link
  8. Liu G, Dou S, Chen X, Chen L, Liu X, Rusckowski M, Hnatowich DJ. Adding a clearing agent to pretargeting does not lower the tumor accumulation of the effector as predicted. Cancer Biother Radiopharm. 2010;25(6):757-62. Link  
  9. Kristjansdottir G, Sandling JK, Bonetti A, Roos IM, Milani L, Wang C, Gustafsdottir SM, Sigurdsson S, Lundmark A, Tienari PJ. Interferon regulatory factor 5 (IRF5) gene variants are associated with multiple sclerosis in three distinct populations. Journal of medical genetics 2008;45(6):362. Link
  10. Fredriksson a, Dixon W, Ji H, Koong AC, Mindrinos M, Davis RW. Multiplexed protein detection by proximity ligation for cancer biomarker validation. Nature Methods 2007;4(4):327-329. Link  
  11. Schallmeiner E, Oksanen E, Ericsson O, Spangberg L, Eriksson S, Ulf-Hakan S, Pettersson K, Landergren U. Sensitive protein detection via triple-binder proximity ligation assays. Nature Methods;2007;4(2):135-137. Link 
  12. Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wählby C, Gullberg M, Botling J, Sjöblom T, Markova B. In situ detection of phosphorylated platelet-derived growth factor receptor using a generalized proximity ligation method. Molecular & Cellular Proteomics 2007;6(9):1500. Link  

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

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   Kit- conjugates 100ug of antibody ($515.00)


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