Antibody-Oligonucleotide All-in-One Conjugation Kit
No chromatography required!
The Application-Proven kit to conjugate antibodies with oligos just got even better.
Download the White Paper
The Antibody-Oligonucleotide All-in-One Conjugation Kit offers an innovative TurboLink™ catalyzed conjugation based on Chromalink™ technology to prepare antibody-oligonucleotide conjugates without chromatography. This novel kit includes everything you need
—buffers, spin columns, and a calculator to determine MSR. No column chromatography is required. With the Antibody-Oligonucleotide All-in-One Conjugation Kit, you can generate high-purity conjugates virtually free of residual antibody or oligonucleotide (>95% conjugate).
Download White Paper: Antibody-Oligonucleotide Conjugate Preparation and Applications
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How it works
Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:
- Amine-modified, 20 to 60-mer oligonucleotide is modified using an excess of the Sulfo-S-4FB linker. This reactive NHS-ester incorporates a 4FB (aromatic aldehyde functional group, formylbenzamide) at the desired terminus of the oligonucleotide.
- Polyclonal or monoclonal antibody (100 μg) is modified using the S-HyNic linker. This NHS-ester reacts with lysine residues, incorporating HyNic functional groups (hydrazino-nicotinamide) onto the antibody.
- The two modified biomolecules are mixed together in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds, followed by magnetic-affinity, solid phase purification.
- The antibody-oligonucleotide conjugate is ready for use in the most demanding and sensitive applications.
- Simple and Easy to Use – Requires only micropipette, microcentrifuge, and UV spectrophotometer
- Automated Calculations – Calculator with fully integrated input/output provided
- High Yield – 30–50% yield based on starting antibody
- High Purity – >95% purity without chromatographic purification
- High Stability – Conjugates are stable for >1 year
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Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.
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