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HL-1002
HyNic Silane


HyNic Silane

The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Chromalink™ technology. This crosslinker is used for incorporation of HyNic linkers on silica/glass surfaces. S-HyNic analog reacts with surfaces, introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers for the immobilization of biomolecules to glass surfaces.

 

Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:  

  • S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
  • S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
  • HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

TriLink Advantages

  • Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
  • Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
  • Stability – 10 times more stable than any other conjugation linker
  • Specificity – Two-linker method avoids homoconjugate formation
  • Efficient – >80% efficient linker-biomolecule conjugations
  • Robust – The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0

 

 

Additional Information

Citations

  1. Luchansky MS, Washburn AL, Martin TA, Iqbal M, Gunn LC, Bailey RC. Characterization of the evanescent field profile and bound mass sensitivity of a label-free silicon photonic microring resonator biosensing platform. Biosensors and Bioelectronics 2010;26(4):1283-91. Link 
  2. Byeon JY, Limpoco FT, and Bailey RC. Efficient Bioconjugation of Protein Capture Agents to Biosensor Surfaces Using Aniline-Catalyzed Hydrazone Ligation. Langmuir, 2010, 26 (19), pp 15430–15435. <NO LINK>
  3. Luchansky MS, Bailey RC. Silicon Photonic Microring Resonators for Quantitative Cytokine Detection and T-Cell Secretion Analysis. Analytical chemistry 2010;82(5):1975-1981. Link 

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

 




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