The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Chromalink™ technology. This crosslinker is used for incorporation of HyNic linkers on silica/glass surfaces. S-HyNic analog reacts with surfaces, introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers for the immobilization of biomolecules to glass surfaces.
Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:
- S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
- S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
- HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.
The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.
- Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
- Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
- Stability – 10 times more stable than any other conjugation linker
- Specificity – Two-linker method avoids homoconjugate formation
- Efficient – >80% efficient linker-biomolecule conjugations
- Robust – The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0
- Luchansky MS, Washburn AL, Martin TA, Iqbal M, Gunn LC, Bailey RC. Characterization of the evanescent field profile and bound mass sensitivity of a label-free silicon photonic microring resonator biosensing platform. Biosensors and Bioelectronics 2010;26(4):1283-91. Link
- Byeon JY, Limpoco FT, and Bailey RC. Efficient Bioconjugation of Protein Capture Agents to Biosensor Surfaces Using Aniline-Catalyzed Hydrazone Ligation. Langmuir, 2010, 26 (19), pp 15430–15435. <NO LINK>
- Luchansky MS, Bailey RC. Silicon Photonic Microring Resonators for Quantitative Cytokine Detection and T-Cell Secretion Analysis. Analytical chemistry 2010;82(5):1975-1981. Link
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