10X TurboLink® Catalyst Buffer is used to catalyze Chromalink® technology, which includes a bond formation between HyNic-modified biomolecules and 4FB-modified biomolecules. In the presence of TurboLink Catalyst the conjugation reaction is complete in 1-2 hours. The active ingredient, aniline, is delivered in 100 mM concentration, a final concentration of 10 mM is sufficient for optimal reaction conditions. This reagent is particularly useful for conjugations of large biomolecules; antibodies, proteins, enzymes, and surfaces.
10X TurboLink Catalyst Buffer is recommended for all HyNic-4FB conjugation reactions and is supplied in the following kits:
- Protein-Protein Conjugation Kit (Cat. No. S-9010-1)
- Protein-Oligonucleotide Conjugation Kit (Cat. No. S-9011-1)
- HRP-Antibody All-in-One Conjugation Kit (Cat. No. A-9002-001)
- AP-Antibody All-in-One Conjugation Kit (Cat. No. A-9105-001)
- R-PE Antibody All-in-One Conjugation Kit (A-9001-006)
- R-PE-Antibody Conjugation Kit (Cat. No. P-9002-002)
Chromalink® technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method for linking any two biomolecules together e.g. proteins, oligos, or peptides regardless of molecular weight.
- S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to biomolecule 1 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 in a catalyzed conjugation reaction. The conjugation time is considerably reduced with the presence of the TurboLink catalyst.
The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm.
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