Protein-Protein Conjugation Kit
The Protein-Protein Conjugation Kit is designed to easily and efficiently conjugate two proteins together. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-protein conjugates to suit their needs. It includes all of the necessary components - including S-HyNic and S-4FB - and protocols for easy and specific crosslinking of any protein with any other protein of a different size greater than 7 kDa.
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Chromalink™ technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method.
- S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to protein 1 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to protein 2 through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- HyNic-modified protein 1 is incubated with 4FB-modified protein 2 in a catalyzed conjugation.
The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm.
The TriLink bioconjugation method, with the addition of the TurboLink™ catalyst, guarantees >95% conversion of protein to conjugate when more than 2 molar equivalents of excess protein is added. High conversion rates, coupled with the unique UV traceable bond formed during crosslinking, allows for easy purification and identification of the conjugate from any excess protein using size exclusion purification methods such as FPLC or diafiltration. Each kit contains the materials to synthesize two conjugation reactions for any ELISA, flow cytometry, IHC, or immunoassay development in just under 4 hours each, yielding between 40–60% conjugate after purification.
Protein-Protein Conjugation Kit Advantages
- Catalyzed conjugation – Faster kinetics for greater efficiency and yields
- Quantifiable – Using a UV signature wavelength and simple UV scan
- Stability – 10 times more stable than any other conjugation linker
- Specificity – Two-linker method avoids homoconjugate formation
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