Protein-Oligo Conjugation Kit
The Protein-Oligo Conjugation Kit is designed to easily and efficiently conjugate two separate protein-oligo conjugates. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-oligo conjugates to suit their needs. It includes all of the necessary components - including S-HyNic and S-4FB - and protocols for easy and specific crosslinking of any protein with any oligo up to 100 base pairs in length.
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Chromalink™ technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method.
- S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to the protein through primary amines (-NH2) on the amino acid, lysine, or on the N-terminus.
- S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to the oligo.
- HyNic-modified protein is incubated with 4FB-modified oligo in a catalyzed conjugation.
The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm.
The TriLink bioconjugation method, with the addition of the TurboLink™ catalyst, guarantees >95% conversion of protein to conjugate when more than 4 molar equivalents of oligo are added. High conversion rates, coupled with the unique UV traceable bond formed during crosslinking, allows for easy purification and identification of the conjugate from the excess oligo using size exclusion purification methods such as FPLC or diafiltration. Each kit contains the material to synthesize two conjugation reactions for any immunoPCR or hybridization-type assay in just under 4 hours each, yielding between 40–60% conjugate after purification.
Protein-Oligo Conjugation Kit Advantages
- Catalyzed conjugation – Faster kinetics for greater efficiency and yields
- Quantifiable – Using a UV signature wavelength and simple UV scan
- Stable – 10 times more stable than any other conjugation linker
- Specificity – Two-linker method avoids homoconjugate formation
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