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How it works
Chromalink™ technology is based on the use of two complementary heterobifunctional linkers:
- S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker, an NHS-ester, reacts with lysine residues, incorporating HyNic functional groups (hydrazinonicotinamide) onto the antibody.
- Sulfo-S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to R-PE, providing a pre-activated, highly fluorescent R-PE called 4FB-R-PE.
- HyNic-modified antibody is incubated with pre-activated 4FB-R-PE in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds.
Catalyzed conjugation. Novel purification.
The efficiency of catalyzed conjugation greatly simplifies conjugate purification. Chromalink™ technology is catalyzed by the TurboLink™ catalyst, aniline, under mild reaction conditions, increasing the rate and efficiency, leading to quantitative conversion of free antibody to conjugate. The complete absence of free antibody at the end of the catalyzed reaction leaves only two components; excess R-PE and conjugate. After conjugation, a novel Q spin filter is used that quantitatively removes excess R-PE to provide high-purity, ready-to use conjugate. Purified conjugate is then eluted from the filter membrane, free of residual antibody and R-PE in high yield (75 μg).
R-PE-Antibody All-in-One Conjugation Kit Advantages
- Catalyzed Conjugation – Fast catalyzed method generates conjugates in just over 6 hr
- Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
- High Purity – Easy spin-column purification columns deliver 100% purified conjugates
- Gentle – Amine-reactive chemistry leaves antibodies intact - no reducing reagents
- Easy-to-Use – Protocols and calculators provided to determine MSR
- Versatile – Compatible with western, ELISA, or IHC applications
- Efficient – 100% conversion with >75% yields
Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.