CleanCap® Reagent AG (3' OMe) is TriLink’s proprietary co-transcriptional capping
reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1
structure. CleanCap has shown to provide up to 98% capping efficiency1.
CleanCap AG (3' OMe) includes the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap AG (N-7113
) has a naturally occurring 5' N7-Methylguanosine structure.
PLEASE NOTE: TriLink recommends using CleanCap AG whenever possible. CleanCap AG requires an AG initiator. CleanCap AG typically provides >90% capped material generating a naturally occurring Cap 1 structure. Unlike CleanCap AG, using CleanCap GG (cat. no. N-7133 or N-7433) may result in comparable ARCA transcriptional stuttering and yield.
Extinction Coefficient: 30,539
Lmol-1cm-1 at 255 nm
Molecular Weight: 1159.6 g/mol (free acid)
Molecular Formula: C33H45N15O24P4 (free acid)
Salt Form: Na+
Purity Specification: ≥95% by AX-HPLC
Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL
Certificate(s) of Analysis
1Final capping is dependent
upon the CleanCap Reagent, DNA template and final mRNA sequence.
Secondary structure due to RNA length and base composition can affect
final capping efficiency.
containing the CleanCap technology are for internal research use only. Not for
use in diagnostic or therapeutic procedures. Use of CleanCap technology
may be covered by one or more patents or pending Patent Applications. www.trilinkbiotech.com/cleancap/license.asp.