CleanCap® Reagent GG (3' OMe) is TriLink’s proprietary co-transcriptional capping
reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1
CleanCap GG (3' OMe) includes the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap GG (N-7133
) has a naturally occurring 5' N7-Methylguanosine structure.
PLEASE NOTE: TriLink recommends using CleanCap AG (cat. no. N-7113 or N-7133) whenever possible. CleanCap AG
requires an AG initiator, whereas CleanCap AG typically provides >90%
capped material generating a naturally occurring Cap 1 structure. Unlike
CleanCap AG, using CleanCap GG may result
in comparable ARCA transcriptional stuttering and yield.
Extinction Coefficient: 37,700
Lmol-1cm-1 at 260 nm
Molecular Weight: 1175.6 g/mol (free acid)
Molecular Formula: C33H45N15O25P4 (free acid)
Salt Form: Ammonium
Purity Specification: ≥95% by AX-HPLC
Shipped at 100 mM in H2O.
1 µmole: 10 µL
5 µmole: 50 µL
10 µmole: 100 µL
Certificate(s) of Analysis
containing the CleanCap technology are for internal research use only. Not for
use in diagnostic or therapeutic procedures. Use of CleanCap technology
may be covered by one or more patents or pending Patent Applications. www.trilinkbiotech.com/cleancap/license.asp.