CleanCap® Cas9 mRNA - (L-7606)

CleanCap® CRISPR Associated Protein 9 mRNA
SKU Unit Size Price Qty
L-7606-20 20 µg
L-7606-100 100 µg
L-7606-1000 1 mg
L-7606-5 5 x 1 mg
Request Bulk Pricing


Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

Cas9 mRNA encodes the Cas9 protein with an N and C terminal nuclear localization signal (NLS). The incorporation of two NLS signals within the mRNA increases the frequency of delivery to the nucleus, thus increasing the rate of DNA cleavage. Additionally, a C terminal HA epitope tag aids detection, isolation, and purification of the Cas9 protein.

This mRNA is capped using CleanCap, our proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Product Details

Catalog No L-7606
Purity Passes Agarose Gel Mobility
Length 4521 nucleotides
Base Composition Unmodified bases
Concentration 1.0 mg/mL
Buffer 1 mM Sodium Citrate pH 6.4
Conversion Factor 40 µg/OD260
Recommended Storage At or below -40°C
Application CRISPR, Immunotherapeutics, Recombinases
Cap AG Start, Cap 1, CleanCap
Other Name(s) CleanCap® CRISPR Associated Protein 9 mRNA

Certificate of Analysis

Intellectual Property

CRISPR/Cas9 License For research use only. Provided with a limited use license under licenses with The Broad Institute and ERS Genomics Ltd.

CleanCap For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at Patent or patent pending,see

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.


Huang, Bonnie; Gomez-Rodriguez, Julio; Preite, Silvia; Garrett, Lisa J.; Harper, Ursula L.; Schwartzberg, Pamela L. . CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development.

Feng, Jifan; Jing, Junjun; Sanchez-Lara, Pedro A.; Bootwalla, Moiz S.; Buckley, Jonathan; Wu, Nancy; Yan, Youzhen; Chai, Yang . Generation and characterization of tamoxifen-inducible Pax9-CreER knock-in mice using CrispR/Cas9.

Kehler, James; Greco, Marianna; Martino, Valentina; Pachiappan, Manickam; Yokoe, Hiroko; Chen, Alice; Yang, Miranda; Auerbach, Jonathan; Jessee, Joel; Gotte, Martin; Milanesi, Luciano; Albertini, Alberto; Bellipanni, Gianfranco; Zucchi, Ileana; Reinbold, Rolland A.; Giordano, Antonio . RNA-Generated and Gene-Edited Induced Pluripotent Stem Cells for Disease Modeling and Therapy.

Peterson, Kevin A.; Beane, Glen L.; Goodwin, Leslie O.; Kutny, Peter M.; Reinholdt, Laura G.; Murray, Stephen A. . CRISPRtools: a flexible computational platform for performing CRISPR/Cas9 experiments in the mouse.

Cunningham, Christopher L.; Wu, Zizhen; Jafari, Aria; Zhao, Bo; Schrode, Kat; Harkins-Perry, Sarah; Lauer, Amanda; Müller, Ulrich . The murine catecholamine methyltransferase mTOMT is essential for mechanotransduction by cochlear hair cells.

Zhang, Xu; Chen, Xiaoli; Liu, Qiuying; Zhang, Shaojie; Hu, Wenqian . Translation repression via modulation of the cytoplasmic poly(A)-binding protein in the inflammatory response.

Brophy, Patrick D.; Rasmussen, Maria; Parida, Mrutyunjaya; Bonde, Greg; Darbro, Benjamin W.; Hong, Xiaojing; Clarke, Jason C.; Peterson, Kevin A.; Denegre, James; Schneider, Michael; Sussman, Caroline R.; Sunde, Lone; Lildballe, Dorte L.; Hertz, Jens Michael; Cornell, Robert A.; Murray, Stephen A.; Manak, J. Robert . A Gene Implicated in Activation of Retinoic Acid Receptor Targets Is a Novel Renal Agenesis Gene in Humans.

Kieffer-Kwon, Kyong-Rim; Nimura, Keisuke; Rao, Suhas S. P.; Xu, Jianliang; Jung, Seolkyoung; Pekowska, Aleksandra; Dose, Marei; Stevens, Evan; Mathe, Ewy; Dong, Peng; Huang, Su-Chen; Ricci, Maria Aurelia; Baranello, Laura; Zheng, Ying; Tomassoni Ardori, Francesco; Resch, Wolfgang; Stavreva, Diana; Nelson, Steevenson; McAndrew, Michael; Casellas, Adriel; Finn, Elizabeth; Gregory, Charles; St Hilaire, Brian Glenn; Johnson, Steven M.; Dubois, Wendy; Cosma, Maria Pia; Batchelor, Eric; Levens, David; Phair, Robert D.; Misteli, Tom; Tessarollo, Lino; Hager, Gordon; Lakadamyali, Melike; Liu, Zhe; Floer, Monique; Shroff, Hari; Aiden, Erez Lieberman; Casellas, Rafael . Myc Regulates Chromatin Decompaction and Nuclear Architecture during B Cell Activation.

Nishino, Kimihiro; Yamamoto, Eiko; Niimi, Kaoru; Sekiya, Yoko; Yamashita, Yoriko; Kikkawa, Fumitaka . N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma.

Kramer, Ashley C.; Blake, Amanda L.; Taisto, Mandy E.; Lehrke, Michael J.; Webber, Beau R.; Lund, Troy C. . Dermatopontin in Bone Marrow Extracellular Matrix Regulates Adherence but Is Dispensable for Murine Hematopoietic Cell Maintenance.

Papizan, James B.; Garry, Glynnis A.; Brezprozvannaya, Svetlana; McAnally, John R.; Bassel-Duby, Rhonda; Liu, Ning; Olson, Eric N. . Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice.

Crawford, Nicholas G.; Kelly, Derek E.; Hansen, Matthew E. B.; Beltrame, Marcia H.; Fan, Shaohua; Bowman, Shanna L.; Jewett, Ethan; Ranciaro, Alessia; Thompson, Simon; Lo, Yancy; Pfeifer, Susanne P.; Jensen, Jeffrey D.; Campbell, Michael C.; Beggs, William; Hormozdiari, Farhad; Mpoloka, Sununguko Wata; Mokone, Gaonyadiwe George; Nyambo, Thomas; Meskel, Dawit Wolde; Belay, Gurja; Haut, Jake; Rothschild, Harriet; Zon, Leonard; Zhou, Yi; Kovacs, Michael A.; Xu, Mai; Zhang, Tongwu; Bishop, Kevin; Sinclair, Jason; Rivas, Cecilia; Elliot, Eugene; Choi, Jiyeon; Li, Shengchao A.; Hicks, Belynda; Burgess, Shawn; Abnet, Christian; Watkins-Chow, Dawn E.; Oceana, Elena; Song, Yun S.; Eskin, Eleazar; Brown, Kevin M.; Marks, Michael S.; Loftus, Stacie K.; Pavan, William J.; Yeager, Meredith; Chanock, Stephen; Tishkoff, Sarah A. . Loci associated with skin pigmentation identified in African populations.

Schuster, Cornelia; Jonas, Franziska; Zhao, Fangzhu; Kissler, Stephan . Peripherally induced regulatory T cells contribute to the control of autoimmune diabetes in the NOD mouse model.

Yin, Hao; Song, Chun-Qing; Suresh, Sneha; Wu, Qiongqiong; Walsh, Stephen; Rhym, Luke Hyunsik; Mintzer, Esther; Bolukbasi, Mehmet Fatih; Zhu, Lihua Julie; Kauffman, Kevin; Mou, Haiwei; Oberholzer, Alicia; Ding, Junmei; Kwan, Suet-Yan; Bogorad, Roman L.; Zatsepin, Timofei; Koteliansky, Victor; Wolfe, Scot A.; Xue, Wen; Langer, Robert; Anderson, Daniel G. . Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing.

Liu, Dajiang J.; Peloso, Gina M.; Yu, Haojie; Butterworth, Adam S.; Wang, Xiao; Mahajan, Anubha; Saleheen, Danish; Emdin, Connor; Alam, Dewan; Alves, Alexessander Couto; Amouyel, Philippe; Di Angelantonio, Emanuele; Arveiler, Dominique; Assimes, Themistocles L.; Auer, Paul L.; Baber, Usman; Ballantyne, Christie M.; Bang, Lia E.; Benn, Marianne; Bis, Joshua C.; Boehnke, Michael; Boerwinkle, Eric; Bork-Jensen, Jette; Bottinger, Erwin P.; Brandslund, Ivan; Brown, Morris; Busonero, Fabio; Caulfield, Mark J.; Chambers, John C.; Chasman, Daniel I.; Chen, Y. Eugene; Chen, Yii-Der Ida; Chowdhury, Rajiv; Christensen, Cramer; Chu, Audrey Y.; Connell, John M.; Cucca, Francesco; Cupples, L. Adrienne; Damrauer, Scott M.; Davies, Gail; Deary, Ian J.; Dedoussis, George; Denny, Joshua C.; Dominiczak, Anna; Dubé, Marie-Pierre; Ebeling, Tapani; Eiriksdottir, Gudny; Esko, Tõnu; Farmaki, Aliki-Eleni; Feitosa, Mary F.; Ferrario, Marco; Ferrieres, Jean; Ford, Ian; Fornage, Myriam; Franks, Paul W.; Frayling, Timothy M.; Frikke-Schmidt, Ruth; Fritsche, Lars G.; Frossard, Philippe; Fuster, Valentin; Ganesh, Santhi K.; Gao, Wei; Garcia, Melissa E.; Gieger, Christian; Giulianini, Franco; Goodarzi, Mark O.; Grallert, Harald; Grarup, Niels; Groop, Leif; Grove, Megan L.; Gudnason, Vilmundur; Hansen, Torben; Harris, Tamara B.; Hayward, Caroline; Hirschhorn, Joel N.; Holmen, Oddgeir L.; Huffman, Jennifer; Huo, Yong; Hveem, Kristian; Jabeen, Sehrish; Jackson, Anne U.; Jakobsdottir, Johanna; Jarvelin, Marjo-Riitta; Jensen, Gorm B.; Jørgensen, Marit E.; Jukema, J. Wouter; Justesen, Johanne M.; Kamstrup, Pia R.; Kanoni, Stavroula; Karpe, Fredrik; Kee, Frank; Khera, Amit V.; Klarin, Derek; Koistinen, Heikki A.; Kooner, Jaspal S.; Kooperberg, Charles; Kuulasmaa, Kari; Kuusisto, Johanna; Laakso, Markku; Lakka, Timo; Langenberg, Claudia; Langsted, Anne; Launer, Lenore J.; Lauritzen, Torsten; Liewald, David C. M.; Lin, Li An; Linneberg, Allan; Loos, Ruth J. F.; Lu, Yingchang; Lu, Xiangfeng; Mägi, Reedik; Malarstig, Anders; Manichaikul, Ani; Manning, Alisa K.; Mäntyselkä, Pekka; Marouli, Eirini; Masca, Nicholas G. D.; Maschio, Andrea; Meigs, James B.; Melander, Olle; Metspalu, Andres; Morris, Andrew P.; Morrison, Alanna C.; Mulas, Antonella; Müller-Nurasyid, Martina; Munroe, Patricia B.; Neville, Matt J.; Nielsen, Jonas B.; Nielsen, Sune F.; Nordestgaard, Børge G.; Ordovas, Jose M.; Mehran, Roxana; O'Donnell, Christoper J.; Orho-Melander, Marju; Molony, Cliona M.; Muntendam, Pieter; Padmanabhan, Sandosh; Palmer, Colin N. A.; Pasko, Dorota; Patel, Aniruddh P.; Pedersen, Oluf; Perola, Markus; Peters, Annette; Pisinger, Charlotta; Pistis, Giorgio; Polasek, Ozren; Poulter, Neil; Psaty, Bruce M.; Rader, Daniel J.; Rasheed, Asif; Rauramaa, Rainer; Reilly, Dermot F.; Reiner, Alex P.; Renström, Frida; Rich, Stephen S.; Ridker, Paul M.; Rioux, John D.; Robertson, Neil R.; Roden, Dan M.; Rotter, Jerome I.; Rudan, Igor; Salomaa, Veikko; Samani, Nilesh J.; Sanna, Serena; Sattar, Naveed; Schmidt, Ellen M.; Scott, Robert A.; Sever, Peter; Sevilla, Raquel S.; Shaffer, Christian M.; Sim, Xueling; Sivapalaratnam, Suthesh; Small, Kerrin S.; Smith, Albert V.; Smith, Blair H.; Somayajula, Sangeetha; Southam, Lorraine; Spector, Timothy D.; Speliotes, Elizabeth K.; Starr, John M.; Stirrups, Kathleen E.; Stitziel, Nathan; Strauch, Konstantin; Stringham, Heather M.; Surendran, Praveen; Tada, Hayato; Tall, Alan R.; Tang, Hua; Tardif, Jean-Claude; Taylor, Kent D.; Trompet, Stella; Tsao, Philip S.; Tuomilehto, Jaakko; Tybjaerg-Hansen, Anne; van Zuydam, Natalie R.; Varbo, Anette; Varga, Tibor V.; Virtamo, Jarmo; Waldenberger, Melanie; Wang, Nan; Wareham, Nick J.; Warren, Helen R.; Weeke, Peter E.; Weinstock, Joshua; Wessel, Jennifer; Wilson, James G.; Wilson, Peter W. F.; Xu, Ming; Yaghootkar, Hanieh; Young, Robin; Zeggini, Eleftheria; Zhang, He; Zheng, Neil S.; Zhang, Weihua; Zhang, Yan; Zhou, Wei; Zhou, Yanhua; Zoledziewska, Magdalena; Howson, Joanna M. M.; Danesh, John; McCarthy, Mark I.; Cowan, Chad A.; Abecasis, Goncalo; Deloukas, Panos; Musunuru, Kiran; Willer, Cristen J.; Kathiresan, Sekar . Exome-wide association study of plasma lipids in >300,000 individuals.

Markossian, Suzy; Guyot, Romain; Richard, Sabine; Teixeira, Marie; Aguilera, Nadine; Bouchet, Mathilde; Plateroti, Michelina; Guan, Wenyue; Gauthier, Karine; Aubert, Denise; Flamant, Frédéric . CRISPR/Cas9 Editing of the Mouse Thra Gene Produces Models with Variable Resistance to Thyroid Hormone.

Johnson, Kenneth R.; Gagnon, Leona H.; Tian, Cong; Longo-Guess, Chantal M.; Low, Benjamin E.; Wiles, Michael V.; Kiernan, Amy E. . Deletion of a Long-Range Dlx5 Enhancer Disrupts Inner Ear Development in Mice.

O'Brien, Lori L.; Guo, Qiuyu; Bahrami-Samani, Emad; Park, Joo-Seop; Hasso, Sean M.; Lee, Young-Jin; Fang, Alan; Kim, Albert D.; Guo, Jinjin; Hong, Trudy M.; Peterson, Kevin A.; Lozanoff, Scott; Raviram, Ramya; Ren, Bing; Fogelgren, Ben; Smith, Andrew D.; Valouev, Anton; McMahon, Andrew P. . Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies.

Webber, Beau R.; O'Connor, Kyle T.; McElmurry, Ron T.; Durgin, Elise N.; Eide, Cindy R.; Lees, Christopher J.; Riddle, Megan J.; Mathews, Wendy E.; Frank, Natasha Y.; Kluth, Mark A.; Ganss, Christoph; Moriarity, Branden S.; Frank, Markus H.; Osborn, Mark J.; Tolar, Jakub . Rapid generation of Col7a1(-/-) mouse model of recessive dystrophic epidermolysis bullosa and partial rescue via immunosuppressive dermal mesenchymal stem cells.

Tröder, Simon E.; Ebert, Lena K.; Butt, Linus; Assenmacher, Sonja; Schermer, Bernhard; Zevnik, Branko . An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.

Redel, Bethany K.; Beaton, Benjamin P.; Spate, Lee D.; Benne, Joshua A.; Murphy, Stephanie L.; O'Gorman, Chad W.; Spate, Anna M.; Prather, Randall S.; Wells, Kevin D. . Single step production of Cas9 mRNA for zygote injection.

Usman, Waqas Muhammad; Pham, Tin Chanh; Kwok, Yuk Yan; Vu, Luyen Tien; Ma, Victor; Peng, Boya; Chan, Yuen San; Wei, Likun; Chin, Siew Mei; Azad, Ajijur; He, Alex Bai-Liang; Leung, Anskar Y. H.; Yang, Mengsu; Shyh-Chang, Ng; Cho, William C.; Shi, Jiahai; Le, Minh T. N. . Efficient RNA drug delivery using red blood cell extracellular vesicles.

Marcus, Assaf; Mao, Amy J.; Lensink-Vasan, Monisha; Wang, LeeAnn; Vance, Russell E.; Raulet, David H. . Tumor-Derived cGAMP Triggers a STING-Mediated Interferon Response in Non-tumor Cells to Activate the NK Cell Response.