CleanCap Reagent GG (3ʹOMe) is designed for the cotranscriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).
CleanCap GG (3' OMe) includes the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap GG (N-7133) has a naturally occurring 5' N7-Methylguanosine structure.
CleanCap can be used in conjunction with TriLink's catalog of modified NTPs.
Using the recommended conditions, transcription with CleanCap GG (3’OMe) results in 70-90% capped material, generating a Cap 1 structure and gives crude yields of ~1.5 mg per mL of transcription. Final capping is dependent upon the DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.
|Purity||≥95% by AX-HPLC|
|Extinction Coefficient||31,700 Lmol-1cm-1 at 255 nm|
|Molecular Formula||C33H45N15O25P4 (free acid)|
|Molecular Weight||1175.7 g/mole (free acid)|
|Recommended Storage||-20°C or below|
|Application||CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development|
|Nucleotide Category||Cap analogs|
CleanCap For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at trilinkbiotech.com/cleancap-research-license.
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
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