CleanCap® Reagent M6 [CleanCap m6AG (3’ OMe)], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base modified Cap 1. Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNAs produced by legacy capping methods. The addition of a methyl group on position 6 of the first adenosine (m6A) may further increase protein expression relative to CleanCap AG or CleanCap AG (3’OMe). It has been hypothesized that the m6A modification adjacent to the 7-methylguanosine cap can positively influence mRNA stability by preventing enzyme-mediated decapping (Mauer et al. 2017)
CleanCap M6 uses the same 5’ AG initiation sequence as CleanCap AG and CleanCap AG (3’OMe).
CleanCap has been shown to provide > 95% capping efficiency and typical crude yields of 4 mg to 5 mg per mL of transcription.
CleanCap M6 can be used in conjunction with wildtype bases or TriLink’s catalog of modified NTPs, such as N1-Methylpseudouridine-5’-Triphosphate (N-1081), Pseudouridine-5’-Triphosphate (N-1019), and 5-Methoxyuridine-5’-Triphosphate (N-1093)
| Catalog No | N-7453 |
|---|---|
| Purity | ≥95% by AX-HPLC |
| Extinction Coefficient | 34,381 Lmol-1cm-1 at 257 nm ± 3 nm in 100mM Sodium Phosphate Buffer pH 7.0 |
| Molecular Formula | C34H48N15O24P4 (free acid) |
| Molecular Weight | 1173.73 g/mole (free acid) |
| Salt Form | Na+ |
| Concentration | 100 mM |
| Buffer | H2O |
| Recommended Storage | -20°C or below |
| Other Name(s) | CleanCap ® Reagent m6AG (3’OMe) for co-transcriptional capping of mRNA, m7(3’OMeG)(5’)ppp(‘5)m6(2’OMeA)pG |
| Application | CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases |
| Backbone | 5'-5'-Triphosphate |
| Base Analog(s) | Adenosine, Guanosine |
| Nucleotide Category | Cap analogs |
CleanCap For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. For additional licensing restrictions, please see the license agreement at trilinkbiotech.com/cleancap-research-license. Patent or patent pending,see trilinkbiotech.com/legal-notices.
Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.
TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser's sole responsibility.
