TriLink’s CleanScribe™ RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter. This enzyme reduces double-strand RNA (dsRNA) levels by up to 85% during IVT. dsRNA is a byproduct of IVT reactions and can trigger undesirable inflammatory responses in host cells.
This novel RNA polymerase can directly substitute a wild-type T7 RNA polymerase in an IVT protocol to synthesize messenger RNAs (mRNAs) from a DNA template. It is highly efficient and specific, capable of transcribing large quantities of RNA in a relatively short time frame. Its robustness, reproducibility, and dsRNA reduction make it ideal for mRNA synthesis as well as other applications such as saRNA synthesis, radiolabeled RNA probe preparation, and RNA construct development for additional studies.
The 100 µg and 840 µg pack sizes (0.2 mg/mL concentration) are configured to run 25 rxns or 250 rxns of 100-μL IVT, following our CleanCap® M6’s co-transcriptional protocol to produce Cap-1 mRNAs. The 1 mg pack size is provided in 1 mg/mL with a separate dilution buffer to optimize enzyme concentrations in your experiments.
The 100 µg, 840 µg, and 1 mg pack sizes contain approximately 50,000 units, 420,000 units, and 500,000 units of enzymes, respectively. One unit of enzyme incorporates 1 nmol of ATP in 1 hour at 37°C.
Learn more about TriLink’s IVT enzymes >
| Catalog No | E-0107 |
|---|---|
| Product Grade | Molecular biology |
| Buffer | 50mM Tris, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 50% glyerol, 0.1% Tween-20, pH 8.0 |
| Reaction Sizes | 25, 250, custom |
| Volume | 0.5 mL, 4.2 mL, 1.0 mL |
| Concentration | 0.2 mg/mL (approx. 100 U/µL), 1.0 mg/mL (approx. 500 U/µL) |
| Host | E. coli |
| Recommended Storage | -15 to -25°C |
ISO 13485 compliant.
Our CleanScribe RNA Polymerase uses the same promoter sequence as a wild-type T7 RNA polymerase, but is not a T7 enzyme.
We are characterizing CleanScribe RNA Polymerase’s performance across a variety of IVT conditions. It shows promising results, and we expect the enzyme to perform well under your conditions.
Yes
Yes, if you don’t linearize, the transcription will continue multiple times and you will not get the expected mRNA product.
