TALEN mRNA Efficiently Generates Transgenic Mice
The gene therapy field has been revolutionized by the introduction of transcription activator-like effector nucleases (TALENs). Though genetic manipulation had long been a tool to delineate gene function, methods were cumbersome and time consuming, until Zinc finger nucleases (ZFNs) were discovered.
ZFNs provided, for the first time, an efficient and relatively simple method for inducing site-specific modifications of genes. However, ZFN design has proven to be difficult and they have been associated with nonspecific cleavage and cytotoxicity. TALENs quickly and efficiently edit genomic DNA in a variety of cell types with greater specificity.